Masters theses : Science

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    Determination of Serum Chromium (Cr), lron (Fe), Copper (Cu), and Cobalt (Co) Levels and Hematological Parameters of People from Blue Nile State, (Engassana Mountains) and Khartoum State
    (ِِAl-neelain University, 2008) Ali Abd Al Ghaffar Abd Al Raheem Mohammed
    In the present study, determination of serum trace elements using Atomic Absorption Spectrophotometry and evaluation of hematological parameters using an Automated Hematology Analyzer in human blood samples of people from Blue Nile State (Bao and Jam areas) and Khartoum State were investigated. Serum elements, Chromium (Cr), Iron (Fe), Copper (Cu) and Cobalt (Co) concentrations and hematological values, hemoglobin (Hb), hematocrit (I-lct), red blood cell counts (RBC), white blood cell counts (WBCs), mean corpuscular volume (MCV), mean cell hemoglobin concentration (MCHC) and mean corpuscular hemoglobin (MCH), concentrations were evaluated regarding sex and location. Chromium and Iron showed significant differences (P< 0.05) in the three different areas however, Iron values were found to be within the 1'10l'lTl8l ranges. Serum levels ofChromium in Jam area showed significant increase (P< 0.05). Copper and Cobalt revealed no significant variations (P>0.05) and the values were found to be in the nonnal reference ranges. Sex had no influence on Chromium, Copper and Cobalt but Iron values were higher in male than female however, the difference was not significant (P>0.05). The hematological values, WBC, RBC, Hb, PCV red cell indices and differential WBC counts showed significant differences (P>0.05) among the three different populations but all of them remained within the normal reference ranges. At the same time all of these values were found to be similar in Bao and Jam areas. The values among sex groups did not show significant differences except for hemoglobin, which was found to be higher in male groups.
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    Potentiality of Using Fungi to Bioremediate Chromium and Lead Contaminated Water
    (Al-Neelain University, 2021) Tasneem Abdelelah Mahmoud Mohamed
    المستخلص تهدف الدراسة لاختبار مقدرة الفطريات علي ازالة الرصاص والكروم من الماء الملوث. استخدام الفطريات لازالة المعادن الثقيله يقلل بتكاليف و ذو فعاليه عاليه مقارنة بالتقنيات الاخري. تم عزل الفطريات باستخدام وسط دكستروز البطاطس المحتوية على رصاص أو كروم بتركيز 50 ميكروجرام/مل. تم اختبار تحمل الفطريات للمعادن الثقيلة في تراكيز مختلفه من الرصاص والكروم بنسبة 0 و 10و 20 و50 و100 ميكرجرام للمل. ثم تم التحضين لمدة اسبوع في ظروف ملائمة. اشارت النتائج الي ان الفطر المعزول لديه القدرة علي ازالة الرصاص والكروم بنسبة 37% للكروم و 50% رصاص. كما اشارت التحاليل الاحصائية الي ان الفطريات امتصت داخل خلاياها 4000% من الكروم و300%من الرصاص. أشارت الخصائص المزرعية والمجهرية إلى أن العزلة تنتمي إلى جنس عفن الخبز Rhizopus spp. تشير هذه النتائج إلى أنه يمكن استخدام الفطريات المعزولة في المعالجة الحيوية لكل من الكروم والرصاص في المياه الملوثة. Abstract The present investigate aimed to study the ability of fungi to remove lead and chromium from polluted water. Fungi were isolated in potato dextrose medium supplemented with lead or chromium at 50 µg/ml concentration. Tolerance to heavy metals was determined by exposing fungi to different concentration of lead and chromium (0, 10, 20, 50, 100 µg/ml). The exposure time was one week under optimum conditions. The result showed that the fungus was able to remove lead (50%) and chromium (37%), and accumulate lead and chromium inside the cells up to 4000% compared to the control. They were able to accumulate lead up to 300% of that of the control. The cultural and microscopic characteristics suggest that the isolate belongs to the genus Rhizopus spp. These results suggest that the isolated fungi can be used for removal of both chromium and lead in polluted water.