Masters theses : Science
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Item Chemical Composition and Anti- Microbial Activity of Leptadenia pyrotechnica and Calotropis procera (Asclepiadaceae) extracts(Al-Neelain University, 2022-08) Soha Fathelrhman Abdalaziz HassanAbstract The aim of this study was to evaluate the effect of Calotropis procera leaf and Leptadenia pyrotechnica aerial parts extracts belonging to the family (Asclepiadaceae) on certain pathogenic microbes, antioxidant activity and it includes extensive chemical study to identify the chemical composition of the active extract by chromatographic and spectroscopic methods. The phytochemical screening of both plant extracts revealed the presence of steroids, glycosides, saponins, tannins and triterpenes. Antimicroial activity were assessed by agar disk diffusion method, results showed that Bacillus subtilis and Staphylococus aurus were the most sensitive to methanol maceration extract of Calotropis procera (19 mm inhibition zone) at the same concentration. Free radical scavenging activity was evaluated using 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical. The overall antioxidant activity of Calotropis proceraand Leptadenia pyrotechnica extracts was found to be the weakest. Based on phytochemial, and antimicrobial activity the methanolic extract of Calotropis procera was subjected to chromatographic and spectroscopic investigations. Column chromatographic fractionation on silica gel packed column was ended up with three fractions. Fraction two from column was further isolated using preparative thin layer chromatography, an afforded five pure compounds which were analyzed by chemical method and analyzed by infra – red (IR) and Ultra violet spectroscopy.Item Chemical Composition and Antimicrobial Analysis of Bosowelliasacra Extract(Al-Neelain University, 2019-07) Afaf Fadollrabo AbdalmajedAbstract The frankincense is an aromatic resin used in incense and perfumes obtained from trees of the genus Boswellia in the family Burseraceae which originated in Arabian peninsula. In this study the preliminary phytochemical screening of (frankincense), methanol, ethanol and water extracts, revealed the presence of flavonoids, terpenoids, steroids, tannins, alkaloids, phenolic compounds, carbohydrates, reducing sugars, deoxy sugars, coumarins, proteins, quinines, anthraquinones, carotenoids, emadols, and saponins. Also quantitative tests to determinant alkaloids (19.4%), flavonoides (85.5%) and saponins (54.4%). From this results it was found that the plant contains high percent of flavonoids. Antimicrobial test was done for each extract to test its activity against four types of bacteria and one fungal. The result have shown that all extract have anti-bacterial activity. In particular, the extracts of petroleum ether and methanol 80% have demonstrated an increased efficacy against the bacteria under study, compared to the other extract. The extracts of frankincense was extracted by maceration extraction method the frankincense revealed that, methanol 80% gave the highest value of total weight in percentage yield (69%), followed by petroleum ether (65.1%), and methanol 80% ( 8.5%). Chemical composition of the chloroform extract was investigated using GC/MS spectroscopy where 25 compounds as hydrocarbons, alcohols, free acids and aliphatic ester. مستخلص البحث اللبان الذكرهو صمغ عطري يستخدم كبخور ويعتبر من النباتات الطبية ويتم إنتاجه من شجرة اللبان والتي تنمو في الهند وشبه الجزيرة العربية . وتشتمل هذه الدراسة على طرق التحليل الكيميائي لمستخلصات اللبان الذكر (الكحولي والمائي) والتي أسفرت عن وجود وفرة في الفلافينويدات ،الاسترويدات ،التانينات ، القلويدات، الجلايكوسيدات، الكينينات، الفينولات، السكريات المختزلة، والمواد الصابونية كما أجريت التجارب الكمية لتحديد نسب القلويدات (19.4%) والفلافينويدات ( 85.5%) والمواد الصابونية ( 54.4%) من خلالها نجد أن هذا النبات يحتوي على نسبة عالية من الفلافينويدات كما أجريت التجارب الحيوية لجميع المستخلصات لإختبار نشاطها كمضادات البكتريا، وأثبتت الدراسة أن المستخلصات لها فعالية ضد البكتريا الإ أن المستخلص الكحولي 80 % له الفعالية الأكثر لكل أنواع البكتريا. وكذلك تم استخلاص النبات عن طريق النقع حيث وجد أعلى عائد من الميثانول النقي (69%) يليه منقوع الايثر البترولي (65.1 %) ثم الميثانول 80% (8.5%) .تم التحقق من التركيب الكيميائي لمستخلص الكحول 80% بواسطة الكروماتوغرافيا الغازية المرفقة بمطيافية الكتلة GC-MS حيث وجد 25 مركباُ مثل الهيدروكربونات والكحول والأحماض الحرة والإستر الأليفاتي.Item Comparative study on the chemical composition and antimicrobial activities of Cymbopogon citratus and Cymbopogon nervatus aerial part extracts(Al-Neelain University, 2021) Nesren Faisal Fadul AbdalgadirABSTRACT This study evaluated the chemical composition and antimicrobial activity of Cymbopogon citratus and Cymbopogon nervatus family (Poaceae). Preliminary phytochemical screening revealed the presence of alkaloids, unsaturated sterols, flavonoids, saponins, coumarins, reducing sugar, cardenolides, and carbohydrates in the ethanol and aqueous extracts of two plants. The aerial part of Cymbopogon citratus and Cymbopogon nervatus were extracted by adifferent methods such as maceration in methanol (80%) and hexane, soxhlet apparatus with several solvents (hexane and methanol) successively and oils were also extracted by steam distillation. The antibacterial activity of the extracts of Cymbopogon citratus and Cymbopogon nervatus were performed by the agar disk diffusion method against gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and one fungi (Candida albicans). The highest mean diameter of the inhibition zone (MDIZ) showed with Cymbopogon citratus oil (31mm) and Cymbopogon nervatus oil (22mm) against Staphylococcus aureus. The physiochemical properties of oils were carried out using standard analytical methods. The density and refractive index of Cymbopogon citratus were (0.8933 g/ml) and (1.487) respectively. The acid value, saponification value, ester value and free fatty acid were in (37.31 mg KOH/g), (74.33 mg KOH/g), (37.03mg KOH/g) and (18.77) respectively. The density and refractive index of Cymbopogon nervatus were (0.9017 g/ml) and (1.491) respectively. The acid value, saponification value, ester value and free fatty acid were in (5.05 mg KOH/g), (95.37 mg KOH/g), (90.32mg KOH/g) and (2.54) respectively. The oils of Cymbopogon citratus and Cymbopogon nervatus were obtained by steam distillation and analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). The oil of Cymbopogon citratus was dominated by monoterpene a-cyclic which accounted for 74.25% of the total oil and characterized by a high percentage of geranial (40.39%) and neral (29.14%). The oil of Cymbopogon nervatus was dominated by monoterpene cyclic which accounted for 85.26% of the total oil and the major compounds are d-Limonene (18.37%). trans-p-Mentha-2,8-dienol (14.78%), Cis-p-Mentha- 1(7), 8- dien- 2- ol (13.72%) and Trans-p-Mentha-1(7), 8-dien-2ol (12.12%). المستخلص في هذه الدراسه تم تقيم المحتوى الكيمائي لنباتي حشيشة الليمون و النال اللذان ينتميا الي العائلة النجيليه وﺩراسـة أﻨﺸـﻁتهما المضادة للميكروبات. تم إجراء المسح الكيميائى النباتى لمستخلصات الماء والايثانول للنباتين والتي أسفرت عن وجوﺩ اللألكلويدات, الإستيرولات غير المشبعه, الفلافينويدات, الصابونيات, الكومارينات, السكريات, الكاردونيليدات والكربوهيدرات. تم استخلاص اجزاء نبات حشيشة الليمون والنال باستخدام طرق مختلفة كالنقع في الميثانول 80% والهكسان، والاستخلاص بجهاز سوكسليت بعدة مذيبات (الهكسان و الميثانول) علي التوالي كما تم استخلاص الزيوت بطريقه التقطير البخاري. ومن ثم أجريت التجارب المعملية الحيوية لكل مستخلصات النباتين لاختبار فعاليتها ضد أربعه انواع من البكتريا المعيارية وهي نوعان موجبة الجرام (العصوية الرقيقة والعنقودية الذهبية ) ونوعين من البكتريا سالبة الجرام (الشريكة القولونية والزائفه الزنجبارية) وفطر المبيضيه البيضاء دلت النتائج أن اعلى متوسط قطر منطقة التثبيط كانت لمستخلص الزيت الطيار لحشيشه الليمون (31 ملم) ولنبات النال (22 ملم) ضد العنقودية الذهبية. ثم تمت دراسة الخواص الفيزيوكيميائية للزيت الطيار لنباتي حشيشه الليمون والنال ووجد ان الثقل النوعى ومعامل الانكسار لنبات حشيشه الليمون (0.8933 g/ml) و (1.487). رقم الحموضة ورقم التصبن ورقم الاستر والاحماض الدهنيه الحره (37.31 mg KOH/g) , (74.33 mg KOH/g), (37.03mg KOH/g), (18.77) على التوالى. الثقل النوعى ومعامل الانكسار لنبات النال (0.9017 g/ml), (1.491). رقم الحموضة ورقم التصبن ورقم الاستر والاحماض الدهنيه الحره (5.05 mg KOH/g), (95.37 mg KOH/g), (90.32mg KOH/g), (2.54) على التوالى وذلك باستخدام طرق قياسية معروفه. كما تم تقدير نوعية وكمية مكونات الزيت لنباتي حشيشه الليمون والنال باستخدام جهاز كروماتوغرافيا الغاز الملحق بمطياف الكتله فوجد أن زيت حشيشه الليمون يحتوى على 74.25% من التربينات الاحاديه المفتوحه وخاصه geranial بنسبة (40.39%)و neral بنسبة (29.14%). كما وجد أن زيت النال يحتوى على 85.26%من التربينات الاحاديه الحلقيه ومن المركبات الاساسيه في زيت النال d- Limonene بنسبة (18.37%) و trans-p-Mentha-2,8-dienol بنسبة (14.78%)و Cis- p- Mentha- 1(7), 8- dien- 2- ol بنسبة (13.72%) و Trans-p-Mentha-1(7), 8-dien-2ol بنسبة (12.12%).Item Investigation of the Chemical Composition of Hydro- Distillated Essential Oil from Sudanese Frankincense (Boswellia papyriferea((Al-Neelain University, 2021-06) Sara Mutasim Mohammed Salih MohammedABSTRACT This research, focused onto instrumental analysis of Hydro-Distillated Essential oil from Sudanese Frankincense Oleo Gum Resins that brought from Darfur Forests, as one of about 20 genus distributed through various regions worldwide. The targeted species is scientifically termed Boswellia papyrifera. The oil color is slightly yellow with specific gravity of 0.9547. The Gas Chromatography-Mass spectroscopy GC-MS was the master depended analytical technique, that provided the study by an accurate qualitative and quantitative data about the chemical composition of the oil of interest, in which octyl acetate appeared as the dominant component of about 82.89%, Cycloheptane-4-methylene-1-methyl-2-(2-methyl-1-propen-1-yl)-1-vinyl 6.20%, 11-Tetradecen-1-ol, acetate 5.15% , 2,6,10-Dodecatrien-1-al,12-(acetoxy)-2,6,10- trimethyl 2.87%, behind another 12 components with less than 1% concentration, the Infrared spectrophotometer IR supported the GC-MS results by confirming the presence of common functional groups in the oil sample i.e. C-H , C=O, and O-H groups at wave number 2856.38 cm -1 , 2956.67 cm -1 sharp peaks ,1743.53 cm -1 , broad 3465.84 cm -1 respectively. From above details, it may be concluded that hydro-distillated essential oil from Boswellia papyrifera oleo gum resins that bring from west Sudanese precisely Darfur consist of various organic compounds either hydrocarbons mostly terpenoids or hydrocarbon derivatives particularly esters. Noted that essential oil extracted from Sudanese Boswellia papyrifera is rich with octyl acetate and other Acetic acid esters that caused the distinctive scent and flavor.نبذة مختصرة ركز هذا البحث على التحليل الآلي للمقطر المائي زيت عطري من راتنجات اللبان السوداني اللبان السوداني غابات دارفور ، كواحدة من حوالي 20 جنسًا موزعة في مناطق مختلفة في جميع أنحاء العالم. يُطلق على الأنواع المستهدفة علميًا اسم Boswellia papyrifera. ال لون الزيت أصفر قليلاً مع الثقل النوعي 0.9547. كان التحليل الطيفي للكتلة والكروماتوغرافيا الغازية GC-MS هو الرائد تعتمد التقنية التحليلية التي زودت الدراسة بنوعية دقيقة والبيانات الكمية حول التركيب الكيميائي للزيت محل الاهتمام ، في التي ظهرت أسيتات الأوكتيل كمكون سائد لحوالي 82.89٪ ، Cycloheptane-4-methylene-1-methyl-2- (2-methyl-1-propen-1-yl) -1- فينيل 6.20٪ ، 11- تتراديسين -1- أول ، أسيتات 5.15٪ ، 2 ، 6 ، 10-دوديكاترين -1 آل ، 12- (أسيتوكسي) -2 ، 6 ، 10- تريميثيل 2.87٪ ، خلف 12 مكوناً بتركيز أقل من 1٪ ، دعم مقياس الطيف الضوئي بالأشعة تحت الحمراء IR نتائج GC-MS من خلال تأكيد وجود مجموعات وظيفية مشتركة في عينة الزيت مثل C-H و C = O و O-H المجموعات عند الموجة رقم 2856.38 سم -1 ، 2956.67 سم -1 قمم حادة ، 1743.53 سم -1 ، واسع 3465.84 سم -1 على التوالي. من التفاصيل أعلاه ، يمكن استنتاج أن الزيت العطري المقطر مائيًا من Boswellia papyrifera راتنجات صمغ الأوليو التي تأتي من غرب السودان تتكون دارفور على وجه التحديد من مركبات عضوية مختلفة إما الهيدروكربونات في الغالب التربينويدات أو مشتقات الهيدروكربون خاصة الإسترات. لاحظ أن الزيت العطري المستخرج من بردية البوزويليا السودانية هو غني بأسيتات الأوكتيل وإسترات حمض الخليك الأخرى التي تسبب الرائحة المميزة ونكهة.Item Chemical Composition and Antioxidant Activity of Solanum dubium ( Gubbain ) Seeds Extracts(Al-Neelain University, 2019-05) Omer Aldaw Hassan MahmodAbstract The aim of this study was to evaluate the effect of the seeds of S.dubium(Gubain) belonging to the family of (solanacea) on, antioxidant activity and it includes extensive chemical study to identify the chemical composition of the seeds by chromatographic and spectroscopic methods A phytochemical investigation was carried out on methanol,ethanol, water,ethanol 95% , and methanol 80% extracts of S.dubium(Gubain, which revealed the presence of saponins ,alkaloids, , flavonoids, tannins, quinine ,glycosides , steroids protein coumarins and phenolic compounds.The volatile oil was extracted from the seeds by water-distillation process. The physicochemical analyses of oil were carried out using standard analytical methods. Results showed that the oil density (0.79 g/cm3), and refractive index (1.47). The acid value (1.610mg KOH/g), saponification number (136.mg KOH/g), and % of free fatty acids (0.80mg KOHg-1 oil), and ester value (134.53) mg KOH/g).The hexane extracts of S.dubium showed a pronounced scavenging activity on DPPH hexane (sox) (94%) and methanol (sox)(93%) at (100mg/ml) concentration. Intensive analytical investigations were carried out on the most active hexanol extract in orderto isolate and identify the effective components using preparative thin layer chromatography (PTLC) that afforded sixth pure fractions were analyzed by Infra-red and Ultra violet spectroscopy. المستخلص الهدف من هذه الدراسة تقييم مدى فعاليه بزور نبات الجبين ودراسة مضادات الأكسدة .ايضآ شملت الدراسة التفصيلية للمحتوى الكيميائي للمستخلصات باستخدام طرق التحليل الكروماتوغرافي والطيفي تم إجراء المسح الكيميائي لمستخلص النبات, ميثانول, ايثانول, 80% ميثانول, 95% ايثانول ومستخلص الماء أسفرت عن وجود, الصابونينات و التنينات, الفلافينويدات, الجلايكوسيدات والكومرينات والمركبات الفينولية استخلص الزيت بطريقة التقطير البخاري ومن ثم تمت دراسة الخواص الفيزيوكيميائية ووجد إن كثافته ( 0.79) ومعامل الانكسار1.47 )) رقم الحموضة( 1.610 Mg KOH/g) رقم لتصبن136 Mg KOH/g)) والأحماض الدهنية الحرة 0.80 Mg KOH /g-1)) وقيمة الاستر 134.53 MgKOH/g)) وذلك باستخدام طرق قياسية معروفة اوضحت نتائج فعالية مضادات الاكسدة أن مستخلص الهكسان سوكسليت (الزيت ) اعطى أعلى تأثير ( 94%) يليه مستخلص الميثانول سوكسليت (93%) عند تركيز 100 mg/ml مقار نتآ ببقية المستخلصات قيد الدراسة تمت دراسة المستخلص الهكسيلي الفعال لمسحوق حبوب الجبين بتقنية كروماتوغرافيا الطبقة الرقيقة التحليلية والتحضيرية التي نتج عنها فصل ستة مجزئات نقية تم تحليلها باستخدام طيف الأشعة تحت الحمراء والأشعة فوق البنفسجيةItem Chemical Composition, Anti-microbial and Anti-oxidant Activities of Rosemary (Rosmarinus officinalis) Leaves Extracts(Neelain University, 2019-04) Mawda Hussein Ibrahim BakhitAbstract Rosemary (Rosmarinus officinalis) is an aromatic shrub belonging to the Lamiaceae family and widely used as herbal remedy and spices, which use to treat various chronic diseases worldwide since ancient times. The preliminary phytochemical screening of ethyl acetate, methanol, and water leaves extracts of Rosemary (Rosmarinus officinalis), revealed the presence of flavonoids, terpenoids, steroids, tannins, alkaloids, phenolic compounds, carbohydrates, reducing sugars, deoxy sugars, coumarins , quinines and carotenoids. The evaluation of chemical constituency of Rosemary leaves found that flavonoids gave (19.5%), tannins (18%), alkaloids (7.1), terpenoids (21%) and saponins (8.5), ash content was (85/100g) and the pH was 6.8. The essential oil of Rosemary leaf was extracted with steam distillation method yielded (1.15%) was analyzed by gas chromatography-mass spectrometry (GC-MS) which revealed the presence of forty two compounds were completely identified and the major compounds were terpenes (71.4%.). The dominant components of oil were Eucalyptol (15.64%), alpha.-Pinene (10.78%). The methanol (mac) extract of Rosmarinus officinalis showed a pronounced scavenging activity on DPPH gave (84%), followed by methanol (sox), (81%), ethyl acetate (68%) and finally water extract (%54) at the same concentration. On the other hand a moderate antibacterial activity was demonstrated by essential oil of rosemary leaf with inhibition zone diameter about (17 mm) against Escherichia coli. The active methanol(mac)extract was further investigated by analytical and preparative TLC chromatography fractionation, afforded nine fractions which were analyzed by spectroscopic methods (Infra-red and Ultra-violet).Item Chemical Composition, Anti-microbial and Anti-oxidant Activities of Rosemary (Rosmarinus officinalis) Leaves Extracts(Neelain University, 2019-04) Mawda Hussein Ibrahim BakhitAbstract Rosemary (Rosmarinus officinalis) is an aromatic shrub belonging to the Lamiaceae family and widely used as herbal remedy and spices, which use to treat various chronic diseases worldwide since ancient times. The preliminary phytochemical screening of ethyl acetate, methanol, and water leaves extracts of Rosemary (Rosmarinus officinalis), revealed the presence of flavonoids, terpenoids, steroids, tannins, alkaloids, phenolic compounds, carbohydrates, reducing sugars, deoxy sugars, coumarins , quinines and carotenoids. The evaluation of chemical constituency of Rosemary leaves found that flavonoids gave (19.5%), tannins (18%), alkaloids (7.1), terpenoids (21%) and saponins (8.5), ash content was (85/100g) and the pH was 6.8. The essential oil of Rosemary leaf was extracted with steam distillation method yielded (1.15%) was analyzed by gas chromatography-mass spectrometry (GC-MS) which revealed the presence of forty two compounds were completely identified and the major compounds were terpenes (71.4%.). The dominant components of oil were Eucalyptol (15.64%), alpha.-Pinene (10.78%). The methanol (mac) extract of Rosmarinus officinalis showed a pronounced scavenging activity on DPPH gave (84%), followed by methanol (sox), (81%), ethyl acetate (68%) and finally water extract (%54) at the same concentration. On the other hand a moderate antibacterial activity was demonstrated by essential oil of rosemary leaf with inhibition zone diameter about (17 mm) against Escherichia coli. The active methanol(mac)extract was further investigated by analytical and preparative TLC chromatography fractionation, afforded nine fractions which were analyzed by spectroscopic methods (Infra-red and Ultra-violet).Item Chemical Composition and Antibacterial Activity of the Volatile Oil of Mentlza Viridis L. Growing in Sudan(Neelain University, 2008) MohiEldin Ahmed Haroun AhmedAbstract- Two samples of Mentha viridis var longifolia were collected in Khartom (central Sudan) and Nyala (Western Sudan) and were investigated for their volatile oils content, their physical and chemical properties, their antibacterial activity and their chemical composition.The two samples were distilled by steam distillation procedure to determine their oil content and it was found that the Khartoum sample contains more volatile oil than the Nyala sample (1.82 and 1.56%) respectively. Physical and chemical properties of the volatile oils of the two samples were determined according to international standards and the results were in agreement with the literature. It was observed that physical parameters of the two samples were almost similar but the acid, ester and saponification values were different. The antibacterial activity of the oils of the two samples were close and moderate. Results of interest were obtained when the volatile oils of the two samples were subjected to GC-MS analysis. The major components as well as the minor ones were different qualitatively and quantitatively.The Khartoum sample contained carvone (46.26%),D-limonene (16.49 %) and dihydrocarveol (12.47 %) as the three major components, but the Nyala sample contained cis-carvone oxide (28.96 %) as the major component in addition to 5-isopropenyl-2-methyl-7-oxabicyclo [4.l.O] heptan-2-ol (16.58 %),2-ethylidene-6-methyl-3,5-heptadienal(l6.25 %) and carveol acetate (11.40 %) as the other major components. It was also observed that the minor constituents were different in the two samples. These findings were reported for the first time in the present thesis and in support of effect of habitat and geographic location on the yield of volatile oils, their physical, chemical and biological properties, as well as their chemical composition.Item Chemical Composition and Antibacterial Activity of the Volatile Oil 0fMentlza Viridis L. Growing in Sudan ‘(Neelain University, 2008) MohiEldin Ahmed Haroun AhmedTwo samples of Mentha viridis var longifolia were collected in Khartom (central Sudan) and Nyala (Western Sudan) and were investigated for their volatile oils content, their physical and chemical properties, their antibacterial activity and their chemical composition.The two samples were distilled by steam distillation procedure to determine their oil content and it was found that the Khartoum sample contains more volatile oil than the Nyala Sample (1.82 and 1.56%) respectively. Physical and chemical properties of the volatile oils of the two samples were determined according to international standards and the results were in agreement with the literature. It was observed that physical parameters of the two samples were almost similar but the acid, ester and saponification values were different. The antibacterial activity of the oils of the two samples were close and moderate. Results of interest were obtained when the volatile oils of the two samples were subjected to GC-MS analysis. The major components as well as the minor ones were different qualitatively and quantitatively.The Khartoum sample contained can/one (46.26%),D-limonene (16.49 %) and dihydrocarveol (12.47 %) as the three major components, but the Nyala sample contained cis-carvone oxide (28.96 %) as the major component in addition to 5-isopropenyl-2-methyl-7-oxabicyclo [4.l.0] heptan-2-01 (16.58 %),2-ethylidene-6-methyl-3,5-heptadienal(16.25 %) and carveol acetate (11.40 %) as the other major components. It was also observed that the minor constituents were different in the two samples. These findings were reported for the first time in the present thesis and in support of effect of habitat and geographic location on the yield of volatile oils, their physical, chemical and biological properties, as well as their chemical composition. I11 . 4Item Chemical Composition and Antioxidant Activity of Ceratonia-siliqua L. pods Extracts(Neelain University, 2018) Adam Ishag Zakaria HassanIn this study, fine powder of the C.siliqua pods (locally known as Algarrob) belongs to family Fabaceae was extracted by soxhlet extraction method using (hexane, chloroform, and, methanol) solvents respectively. Preliminary phytochemical screening of ethanol and water extracts of C.siliqua pod which indicated the presence of flavonoids, sterols, triterpenes, alkaloids, saponins, tannins, and leucoanthocyanins. The elements composition of C.siliqua carob pods was evaluated using Atomic Absorption Spectrometry (AAS). The results obtained revealed that concentration of metals were Na (172), Zn (117.1), Mn (11.6) in ppm. The moisture and ash content of C.siliqua pods are found to be (4.4 %) and (19.47%) respectively. The C.siliqua pod was evaluated for their total flavonoids were represented (18.1%) tannins (14.4%), alkaloids, (6.18%) saponins (4.5%) and trepenes (3.03%) contents, while the antioxidant activity was assessed in vitro using DPPH radical scavenging assays. The methanolic extract of C.siliqua contained the highest flavonoid and tannins and subsequently showed a pronounced scavenging activity on DPPH (92%) inhibition at (100 mg/ml) concentration. Intensive analytical and spectroscopic investigations were carried out on the most active methanolic extract in order to isolate and identify the effective antioxidant component using analytical and preparative thin layer chromatography, finally analyzed by Infra-red and Ultra-violet spectroscopy. The results led to the isolation, purification and characterization of eight pure fractions. المستخلص تناولت هذه الدراسة استخلاص مسحوق ثمارالخروب المتوفر بالسودان والذى ينتمى الى عائلة القرنية بطريقة الإستخلاص المستمر (سوكسليت) بالمذيبات (الهكسان الكلورفورم والميثانول) على التوالي. كما تم إجراء المسح الكيميائي النباتي لمستخلص الايثانول والمستخلص المائى(نقع) الذى أسفرعن وجود الفلافونيدات, الأستيرولات, التربينات، القلويدات, الصابونينات, التانينات وليكوانثوسيانينات. تم تحديد تركيز بعض العناصر باستخدام جهاز مطياف الامتصاص الذري AAS) ). وقد بينت نتائج الدراسة ان تراكيز العناصر في العينة كانت كما يلي: الصوديوم (172.1) , الزنك (117.1) , والمنجنيز)11.6) بوحدة الجزء من المليون ،.بينت النتائج ان قيمة الرطوبة والرماد فى ثمار نبات الخروب (4.4%), (19.49%) على التوالى. كما بين التقدير الكمى لمسحوق ثمارالخروب ان الفلافنويدات تمثل(18.1%) التانينات(14.4%) القلويدات(6.18%) والتربينات (3.03%) . ثم أجريت التجارب المعملية لمستخلص الميثانول لاختبار فعاليتة كمضاد للاكسدة ، أوضحت النتائج ان لمستخلص الميثانول تأثير عالى 92%)) عند تركيز100mg/ml. عليه أخضع مستخلص الميثانول الفعال لمسحوق ثمار نبات الخروب لفعاليته العالية ضد الاكسدة لعملية التجزئة بواسطة كروماتوغرافيا الطبقة الرقيقة التحليلية و التحضيرية, نتج عنها الحصول على ثمانية مجزئات نقيه تم تحليلها بواسطة طيف الأشعة تحت الحمراء والأشعة فوق البنفسجية