Masters theses : Science

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    Isolation and identification of aetiological agent of middle ear infection (otitis media) and their antibiotic susceptibility in Khartoum state.
    (2011) Amira Shareif Omer Tia
    Abstract This study was done to investigate the occurrence of the middle ear infections among different ages and gender, besides isolation and identification of the causative aerobic bacteria and fungi that associated with middle ear infections and also to evaluate the antimicrobial activity with certain antibiotics against the isolated bacteria. One hundred samples were collected from Ear Nose Throat (ENT) Hospital of Khartoum. The causative bacterial agents were isolated by using different types of culture media and identified by using biochemical tests. Antimicrobial susceptibility tests for bacteria were performed using modified Kirby Bauer disc diffusion method, and using national committee for clinical laboratory standards (N CCLS). Fungi were isolated and identified by using culture media and examined microscopical examination. Gram-positive bacteria that were isolated in this -study included Staphylococcus aureus (6.4%), Staphylococcus epidermidis (1.8%), Staphylococcus sabrophyiieus (0.9%) Aerococcus viridiarzs (ll.8%), Listeria monocytogenes (0.9%) Bacillus badius (0.9%) ‘Bacillus megatarium (0.9%) and Corynebacterium spp (0.9%). Gram -negative bacteria which were isolated included Aeromonas hydrophila (16.4%), Pseudomonas aeruginosa (4.6%), Proteus mirabilis(3.6%), Proteus vulgaris (1.8%); Proteus rettgeri (1.8%), Proteus vulgaris biogroup2(l.8%), Haemophillus influenza(l.8%), Erwinia herbicola (0.9%), Pseudomonas spp(5.5%) Acaligenes spp(l.8%), Citrobacter fieundii (0.9%), Klebsiella spp(3.6%), Acirietobacter spp(4.6%), Chromobacterium vi0laceium(6.4%). Enterobacter aerogenes , (0.9%), Actinobacillus spp (0.9%), Aerobacter spp (0.9%) and Escherichia fergusonii (0.9%). Antimicrobial susceptibility tests showed that Gram positive bacteria includes Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus sabrophyiticus, Listeria monocytogenes , Aerococcus virialians, Bacillus