l-IAEMOSTATIG ABNORMkL.I'TlES AKMONG A suimnasa woman wma unaxamman l RECURRENT ABORTION Y

Abstract

Background: Hereditary thrombophilias, such as deficiencies of natural anticoagulants, as well as common thrombotic genetic mutations are well documented as causes of recurrent spontaneous abortion. _ Objective: This study aimed to screen for both common thrombotic genetic mutations and coagulation inhibitors deficiency related 'to recurrent pregnancy loss among Sudanese women. ‘ Materials and methods: the study is a case control study, included 100 Sudanese women with unexplained recurrent pregnancy loss, and 50 healthy, age-matched reproductive Sudanese women as a control group. Two blood samples were collected fiom each subject, one in 3.2% tri-sodium citrate for coagulation investigations and the other in ethylene diamine tetra acetic acid (EDTA) for molecular analysis. _ Protein C, protein S, and antithrombin HI were measured by chromogenic assay. Genomic DNA was extracted from EDTA samples and analysed for the presence of factor V Leiden mutation, prothrombin G20210A mutation, and methylene tetrahydrofolate reductase mutation using polymerase chain reaction- restriction fiagment polymorphism (PCR-RFLP). XVI"