Masters theses : Medicine

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    Molcular Characterization of Extended Spectrum Beta Lactamaese Producing Klebsiella Pneumoniae Clinical Isolates in Khartoum state
    (AL-Neelain University, 2020) Abdah Abdelmonim Adam Azrag
    Background: The emergence of extended-spectrum [3-lactamases (ESBLs)- producing bacteria, particularly Klebsiella pneumoniae, is now on a critical concem for the development of therapies against bacterial infections specially for healthcare associate infections caused by such Multi-Drug Resistance(MDR) and super bug G- ve bacterial pathogens. Aim of the study: Current study aimed to detemiine the prevalence of Extended Spectrum Betalactamases producing genes in Klebsiella. pneumoniae isolates from Khartoum Hospitals. Materials and methods: This is hospitals-based descriptive cross sectionl study conducted in Khartoum, after it had been approved form AL neelain University, Sudan. Out of four hundred clinical swabs samples collected from different Khartoum hospitals, 56 identified clinical isolates of Kpneumoniae the antibiogram of these isolates assessed phenotyically against eight antimicrobials, as well as genotypicaly for the prevalence and distribution of three ESBL genes which were SHV, TEM and CTX using multiplex PCR assay. Results: Out of fifty six identified clinical isolates of K.Pneum0niae the phenotypic antibiogram was assessed, and the prevalence of ESBLs was found to be 75%. The percentage of genotypic distributions to all confirmed ESBL Klebsiella .pneum0niae were determined via Multiplex PCR assay for three ESBL genes which were TEM gene lOO%, 95% for SHV gene and 17% to CTX gene. Conclusion: This study concluded that the TEM gene has a highest prevalence in ESBL producing Kpneumoniae followed by CHV, and CTX. iv