Masters theses : Medical Laboratory Science

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    The Histopathology of African Horse Sickness Virus in Suckling Mice
    (Al Neelain University, 2017-01) NahidAbdallaGasemElseed
    This investigation aimed to study the histopathological changes of AHSV in suckling mice in predilection sites (skin, heart and lungs). African horse sickness virus vaccine was obtained from Central Veterinary Research Laboratory (CVRL) (Khartoum, Sudan) in form of freeze-dried vaccine vial. An amount of 0.02 ml of virus was inoculated intra-cerebrally in 1-7 days old conventional laboratory albino mice. The inoculation was done in three separate times.The numbers of mice inoculated were 8 mice; three days old inoculated with 10-4 of the virus, 4 mice; four days old inoculated with 10-2 of the virus and 5 mice; 6 days old inoculated with 10-2 of the virusin first, second and third inoculation, respectively. In each inoculation tow mice were inoculated intra-cerebrally with 0.02 ml of normal saline and kept as control. The inoculated mice became apparently sick and developed singes of encephalitis, difficulty in breathing with mouth open and head hanging down, Oedema,congestionand hemorrhage particularly in the abdominal area and at extremities was observed in all of them.Histopathological investigation showedsinges of infection in the form of inflammatory changes including: Oedema, hemorrhages and leukocytes infiltration in all selected organs (lungs, heart and skin and subcutaneous tissues).The similarity of sings, symptoms and the histopathological lesions between the mice and the original host indicated that mice are suitable experimental animal model to study the pathogenesis of AHSV.