Browsing by Author "Ahmed Bolad"

Now showing 1 - 17 of 17

Antibody And Cellular Immune Responses In Individuals Living In Areas With Insecticde Treated Bed Nets
(Nile Basin Studies and Research Center, 2008-11) Ahmed Bolad
Antibody-mediated in vitro growth inhibition of field isolates of Plasmodium falciparum from asymptomatic children in Burkina Faso
(The American Society of Tropical Medicine and Hygiene, 2003-06-01) Ahmed Bolad
Antibody-mediated inhibition of Plasmodium falciparum parasites in vitro reflects the potential parasite-neutralizing activity of the antibodies in vivo. In this study, immunoglobulins and P. falciparum isolates were collected from children with asymptomatic malaria in Burkina Faso. We demonstrate a significantly lower in vitro growth inhibitory activity against the P. falciparum field isolates by autologous host immunoglobulin compared with that of immunoglobulin from other individuals. To gain further insight to possible ...
Assessment of Nurse’s Competences during Emergency Management of patients with Acute Myocardial Infarction at critical care units and emergency department at Public Teaching Hospitals in Khartoum State (2013
(جامعة النيلين- مركز النيلين الطبي, 2013) Hassanat Elbashir Mohamed Mustafa1; Mustafa Nimeiri; Ahmed Bolad; Siddig Khalil1
Clarithromycin-Resistant Helicobacter Pylori Strains among Dyspeptic Patients in Sudan
(جامعة النيلين- مركز النيلين الطبي, 2012) Nazar Abdalazeem; Hassan Abdul-Aziz; Adam Ahmed Adam; Waleed Hussein Omer; Ahmed Bolad
The clinical importance of anti-CCP in early diagnosis of Sudanese patients with rheumatoid arthritis
(جامعة النيلين - مركز النيلين الطبي, 2015-11) Ahmed Bolad
Abstract Background: Rheumatoid Arthritis (RA) is a systemic inflammatory autoimmune disorder; it is the most common inflammatory arthritis in the world. The early diagnosis and effective treatment of rheumatoid arthritis (RA) can improve the clinical outcome of the disease. Recently, anti-Cyclic- Citrullinated protein antibodies (Anti-CCP) are found to be more specific to RA arthritis although in the early stages when the arthritis is undifferentiated. The objective of this study was to determine the clinical importance and the prevalence of anti-CCP in early diagnosis of Sudanese patients with RA. Methodology This work was a cross-sectional study conducted at Al Ribat teaching hospital (from June 2012 to ? 2014). The study sample included 56 Sudanese patients, known patients of RA who fulfilled the ACR criteria. Demographic and clinical data were collected by questionnaires. All patients and controls sera were investigated for anti-CPP by enzyme linked immunosorbent assay (ELISA). Results The mean age of RA patients in this study was 46.0 (±11.7) years. The control group consisted of 56 Sudanese apparently healthy subjects, 27 (48%) of them were female with mean age of 37 ±17.2years. The anti-CCP antibodies were detected in 60.7% of cases, and in two (3.6%) of the control group. The sensitivity of anti-CCP was 60.7% and specificity of 96.4%. Anti-CCP test has a maximum proportionate reduction in uncertainty (PRU) of 94% for a positive result and 59% for a negative result. Conclusions Anti-CCP antibody has a higher diagnostic specificity and positive predictive value than rheumatoid factor; however its sensitivity was low. The consent of each patient was obtained prior to commencement of data collection.
The clinical importance of anti-CCP in early diagnosis of Sudanese patients with rheumatoid arthritis
(جامعة النيلين - كلية الدراسات العليا, 2014-12-22) Yousif Osman; Shaaban Khudair; Ahmed Bolad
Background: Rheumatoid Arthritis (RA) is a systemic inflammatory autoimmune disorder; it is the most common inflammatory arthritis in the world. The early diagnosis and effective treatment of rheumatoid arthritis (RA) can improve the clinical outcome of the disease. Recently, anti-Cyclic-Citrullinated protein antibodies (Anti-CCP) are found to be more specific to RA arthritis although in the early stages when the arthritis is undifferentiated. The objective of this study was to determine the clinical importance and the prevalence of anti-CCP in early diagnosis of Sudanese patients with RA. Methodology This work was a cross-sectional study conducted at Al Ribat teaching hospital (from June 2012 to ? 2014). The study sample included 56 Sudanese patients, known patients of RA who fulfilled the ACR criteria. Demographic and clinical data were collected by questionnaires. All patients and controls sera were investigated for anti-CPP by enzyme linked immunosorbent assay (ELISA). Results The mean age of RA patients in this study was 46.0 (±11.7) years. The control group consisted of 56 Sudanese apparently healthy subjects, 27 (48%) of them were female with mean age of 37 ±17.2years. The anti-CCP antibodies were detected in 60.7% of cases, and in two (3.6%) of the control group. The sensitivity of anti-CCP was 60.7% and specificity of 96.4%. Anti-CCP test has a maximum proportionate reduction in uncertainty (PRU) of 94% for a positive result and 59% for a negative result. Conclusions Anti-CCP antibody has a higher diagnostic specificity and positive predictive value than rheumatoid factor; however its sensitivity was low.
Comparison of Rheumatoid Factor and anti-Cyclic-Citrullinated protein antibodies for the Diagnosis of rheumatic arthritis in Khartoum, Sudan
(جامعة النيلين, 2008) Shaaban Khudair; Mohammed Abbas; Ahmed Bolad; Mowahib Al Edressy
Comparison of Rheumatoid Factor and anti-Cyclic-Citrullinated protein antibodies for the Diagnosis of rheumatic arthritis in Khartoum, Sudan
(جامعة النيلين - كلية الدراسات العليا, 2014-12-22) Shaaban Khudair; Mowahib Al Edressy; Mohammed Abbas; Ahmed Bolad
Background: The objective of the current study was to determine the sensitivity and specificity of anti-Cyclic-Citrullinated protein antibodies (anti-CCP antibodies) as compared to that of Rheumatoid Factor (RF) in diagnosing patients with rheumatoid arthritis (RA) Methodology Fifty six samples were collected from Sudanese patients (46 females, 9 males) with rheumatic diseases who visited the rheumatology clinic ElRibat Hospital, Khartoum, Sudan. Titers of RF and anti-CCP antibodies of each patient were recorded. Sensitivity and specificity of the test were evaluated using ELISA as the gold standard method. Results The sensitivity of (RF) test (41/56) was 73.2% whereas the sensitivity of Anti CCP test (34/56) was 60.7%. The specificity of RF test (44/56) was 78.6%, whereas the specificity of Anti CCP test (54/56) was 96.4%. Conclusions The combination of anti-CCP and RF tests provides nearly 100% and thus could be helpful in the differential diagnosis of RA and other rheumatic diseases
Culturing of erythrocytic asexual stages of Plasmodium falciparum and P. vivax
(Methods in malaria research, 2008) Fingani Mphande, Sandra Nilsson; Ahmed Bolad
Comment: For growing parasites from patient blood, use 10 g of Albumax for 1 liter of complete MCM. The vast majority of cultures will survive at least 2 weeks. It is also important to avoid serum in the culture for preparation of crude parasite antigen (see SEROLOGY, section III: B). Not all strains can be adopted to Albumax II medium.
Distinct interethnic differences in IgG class/subclass and IgM antibody responses to malaria antigens but not in IgG responses to non-malarial antigens in sympatric tribes living in West Africa
(Scandinavian Journal of Immunology, 2005) Ahmed Bolad
The well-established relative resistance to malaria observed in the Fulani as compared with other sympatric tribes in West Africa has been attributed to their higher levels of serum immunoglobulin (Ig) G antibodies to malarial antigens. In this study, we confirm and extend the previous findings by analyses of the levels of IgM, IgG and IgG subclasses of anti-malarial antibodies in asymptomatic individuals of different sympatric tribes in Burkina Faso (Fulani/Mossi) and Mali (Fulani/Dogon). The Fulani showed significantly higher median concentrations of anti-malarial IgG and IgM antibodies than the sympatric tribes at both locations. Although the overall subclass pattern of antibodies did not differ between the tribes, with IgG1 and IgG3 as dominant, the Fulani showed consistently significantly higher levels of these subclasses as compared with those of the non-Fulani individuals. No significant differences were seen in the levels of total IgG between the tribes, but the Fulani showed significantly higher levels of total IgM than their neighbours in both countries. While the antibody levels to some nonmalarial antigens showed the same pattern of differences seen for antibody levels to malaria antigens, no significant such differences were seen with antibodies to other nonmalarial antigens. In conclusion, our results show that the Fulani in two different countries show higher levels of anti-malarial antibodies than sympatric tribes, and this appears not to be a reflection of a general hyper-reactivity in the Fulani.
Family and Community Support of TB Patients -Jebel Awleyah Locality-Khartoum Sudan -2011
(جامعة النيلين, 2012) Mustafa Khidir Mustafa Elnimeiri; Ahmed Bolad
Family and community support are always considered as essential factors need to facilitate coping of the patients with their disease and its outcome. In Sudan the family and ties are still firm. However family and community support for TB patients may not always be encountered to the same extent especially among urban populations. The design was facility-based descriptive. The study was conducted in Gebel Awleyah Locality, which is geographically located in the South of the National Capital of Sudan. The study population was composed of 348 TB patients (pulmonary and extra-pulmonary) in the TBMUs receiving DOT in the intensive phase. A Standardized pre-coded and pre tested administered questionnaire was used for data collection from the TB patients. 99% of the TB patients were supported by their families as they became sick. The family support was expressed in accompanying to the TBMU (96%) and in provision of extra food (99%). The family support in cash was encountered for about 73% of the TB patients. About 31% of the TB patients were supported by the community and 69% of them were not supported. For those TB patients, who were subjected to community support, were mainly supported by their neighbors (98%). The TB patients of both genders were supported by their families mainly by their bothers. The family support was marked in accompanying to the TBMUs and provision of extra food.
Glutamate-rich protein (GLURP) induces antibodies that inhibit in vitro growth of Plasmodium falciparum in a phase 1 malaria vaccine trial
(Elsevier, 2007) Ahmed Bolad
The glutamate-rich protein (GLURP) of P. falciparum is the target of cytophilic antibodies which are significantly associated with protection against clinical malaria. A phase 1 clinical trial was conducted in healthy adult volunteers with the long synthetic peptide (LSP) GLURP85–213 combined with either Aluminum Hydroxide (Alum, 18 volunteers) or Montanide ISA 720 (ISA, 18 volunteers) as adjuvants. Immunizations with 10, 30 or 100μg GLURP85–213 were administered subcutaneously at days 0, 30, and 120. Adverse ...
Operational Research from Theory to Practice October 2011
(جامعة النيلين- مركز النيلين الطبي, 2013) Mustafa Khidir Mustafa Elnimeiri; Ahmed Bolad
Prevalence of Schistosomiasis among School Children-South Kordofan State
(جامعة النيلين- مركز النيلين الطبي, 2012) Mustafa Khidir Mustafa Elnimeiri; Ahmed Bolad
THE PREVELANCE OF HELICOBACTER PYLORI AMONG PATIENTS WITH CHRONIC IDOPATHIC URTICARIA
(مركز النيلين الطبي, 2015) Sheema Mohsin Mustafa; Ahmed Bolad
Literature review: Helicobacter pylori was first isolated by Warren and Marshall (Warren et al., 1983) in 1982 it was linked with chronic antral gastritis and peptic ulceration, at first they named the bacterium as Campylobacter pyloridis, later on it was renamed as Helicobacter pylori (Warren et al. 1984). The genus Helicobacter belongs to the subdivision of the Proteobacteria, order Campylobacterales, family Helicobacter-aceae. The genus Helicobacter consists of over 20 recognized species (Fox, 2002). Members of the genus Helicobacter are all microaerophilic organisms and in most cases are catalase and oxidase positive, and many but not all species are also ureases positive. Helicobacter species can be subdivided into two major lineages, the gastric Helicobacter species and the enterohepatic (nongastric) Helicobacter species. Both groups demonstrate a high level of organ specificity, such that gastric helicobacters in general are unable to colonize the intestine or liver, and vice-versa.
Rheumatoid arthritis and anti-CCP
(جامعة النيلين - كلية الدراسات العليا, 2014-12-22) Yousif Osman; Shaaban Khudair; Ahmed Bolad
Rheumatoid arthritis and anti-CCP
(جامعة النيلين, 2010) Yousif Osman; Shaaban Khudair; Ahmed Bolad
Rheumatoid Arthritis (RA) is a systemic inflammatory autoimmune disorder characterized by widespread joint inflammation with synovial hyperplasia disease and the most common inflammatory arthritis, affecting from 0.5 to 1% of the general population worldwide (Gabriel, 2001). It is more common in women with female to male ratio of 2 to 4.1 (Gabriel, 1999). The peak incidence between 30 and 50 years (Ceccato et al., 2006