The effects of hyperthyroidism and hypothyroidism hormones on gonadotrophins and lipids profile in female Wistar rats

Abstract

10

Abstract

Thyroxine and tri

-

i

odothyronine are essential for normal organs growth, development and

functions. These hormones regulate the basal metabolic rate (BMR) of all cells and play a

critical role in the development of several organs systems, include the reproductivesystem. This

study focuses on the effects of iodothyronines

on plasma lipids and FSH, LH concentrations in

female Wistar rats and answer specific questions, what is worse to have low or excess thyroid

hormones in female Wistar rats. Adult female Wister rats

(number 48

), these divided into two

main groups

hyperthyroidism and hypothyroidism forty eight

rats in each group. The two main

groups devided into 12 rats as treated and 12 rats as control (euthyroid). The experimental

study was located at the UKZN, Faculty of Sci

ence and Agriculture School of Biochemistry,

Genetics and Microbiology, University of Kwa Zulu

-

Natal, Westville campus, South Africa

from October 2009 up to May 2010. The rats became

hyperthyroidism

by administrating

thyroxine orally 90

-

100 μg/kg b.w for 2

1 days or hypothyroid

ism

by administrating

carbimazole orally 50 mg/kg b.w for 21 days, euthyroid received 3 ml of deionized water. The

rats firstly were put in the metabolic cage 24 hours for three days for acclimatized. On day one

of experimental base l

ine rats were put in metabolic cages for 24 hours. Every third day of

experimental rats weight, water and food consumption, urine production and blood pressure

were recorded. On day 23 all rats were sacrificed from both groups treated and control. Blood

sa

mples were taken directly from the right atrium for analysis of thyroid hormones TSH,

T

3

and T

4

gonadotrophins FSH,LH lipids profile (total cholesterol, triglycerides and HDL

-

cholesterol) and total proteins. Also organs were dissected out and weights inclu

de liver,

thyroid gland,

spleen

and ovaries from all animals. Thyroid and gonadotrophins hormones were

measured by using of enzyme immunoassay kits from (

TOSOH, Corporation

Japan). Lipids

profile and total proteins were measured by using commercial kits f

rom BioSystem S.A. Costa

Brava 30, Bracelona (Spain), by the Hitach 906 analyzer. The results of body weight in

hyperthyroid

ism

female rats group showed significant

decrease (P

≤ 0.05), and these results

were associated with reduction in food and water consumption compared to control rats group

.

The results of

T

3

concentrations

showed

in hyperthyroid

ism female Wistar

rats group

showed

significant

(P

≤ 0.05)

inc

reased, but T

4

showed

highly

significant

(P

≤ 0.05)

increased

compared to control group

.

The

results of gonadotr

o

phins

in both treated groups

showed conflict results

.

The results of lipid

s

profile in hyperthyroidism rats (TC and HDL

-

C)

11

showed

decreased

not significant

,

but TG

was

significantly decreased

(P

≤ 0.05)

compared to

control group

.

H

ypothyroidism rats group showed

for both (TC and TG)

showed increased not

significant, but HDL

-

C w

as

significantly (p <0.05) increased

compared to control group

.

Thyroid gland, liver and ovaries were removed from hyperthyroidism and hypothyroidism

female Wistar rats and control for histopathological examination under light microscope. Nine

glass slides

were

examined by light microscopy. The examination results obse

rved there is

change in thyroid gland cells which collapsed in hypothyroidism and converted to large

follicular collid in hyperthyroidism and ovaries of tow treated group due to the effect thyroxine

and carbmizole used in inducing two conditions.

From th

is study we published two papers the first (The effects of Induced Hyperthyroidism on

plasma FSH and LH Concentration in Female of Wistar Rats )

in Research Journal of Recent

Science Vol.1(6),55

-

57,June (2012) the second p

aper ( the effect of carbmaizo

le induced

hypothyroidism on plasma lipids profile concentration in female Wistar rats)

in the University

of Dongola Journal for Scientific Research, Third Year Issue No.4

–

January

-

2013

.