micro determinatino of chloride ino using an amplification method

Abstract

summary The published methods for the applications of amplification reaction in the analysis of various compounds have been reviewed. In this study the indirect amplification technique was used to estimate microgram amount of chloride ions. Three solid iodates (AgIO3, Hg(IO3)2 and Hg2(IO3)2 ) were tested for their relative efficiency in metathesis reaction with chloride ion. The best results were obtained with AgIO3. The effect of temperature, amount of solid iodate and acidity using HNO3 on the indirect amplification procedure (metathesis) were examined. The constant percentage conversion of chloride ion to iodate form allowed a quantitative measurement of this ion to be made. Aliphatic chloro-compounds (e.g chloroform) are hydrolyzed readily under the alkaline condition and releasing chloride ion. The development and refinement of a method for the determination of chloroform using amplification procedure is described in this thesis. The concentration of the chloroform is determined by titration of the released chloride and the percentage recovery was 100.42 %. RSD = 1.59 % (n=4).