Antimicrobial Activity of Trachyspermum ammi Against Some Clinical Isolates

Abstract

This study was carried out in Khartoum Teaching Hospital and Department of Microbiology and Molecular Biology, Faculty of Science and Technology, EL Neelain University. Evaluation of possible antimicrobial activity of »Tracl1y_spermmn ammi, also known as (Carum copticmu, Carum ajowan, Trachyspcrmum coptjcum) seeds extracts in diflerent concentrations against Slap/1yl0c0ccu.s' aureus, /5.00!/', I’r0tuus vu/gar/'.\', 1’.s'eua'0m0na.r aerugi/1,0.s'a and Candida al/Jicans was made. These organisms were isolated from dillierent specimens (ear and eye swabs, urine, wounds). Out of I34 collected specimens, .\'Iu/1/iy/000ccu.\' uu['cu.\' represented a high incidence 5‘) (44%), whereas (Yum//1/u 1///liar/n.\' showed lower incidence 9 (7%) The plant seeds were extracted successively using petroleum ether, clilorolbrm, ethanol and'water extracts, and the extracts were tested for activity against isolated microorganisms. I lt was observed that petroleum ether and ethanol extracts from the plant seeds were active; the aqueous and chloroform extracts were, however, less active. This might be due to the presence and concentration 0|" active constituents more extractable with petroleum ether and ethanol than wzrer or chlorol'orm. The antimicrobial activity ol' the plant extracts were compared with the activity ol‘the antibiotic gentamicin. _ Phytochemieal analysis ot"the plant seeds demonstrated the presence ol alkaloids, flavone aglycones and eoumarins in petroleum ether extract, anthraquinones, eoumarins, anthrasine glyeosidcs and condensed tannins in alcohol fraction and triterpene glyeosides, saponins, alkaloids and condensed tannins in aqueous extract