Antimicrobial And Wound Healing Activity Of Plicosepalus Acaciae (ZuCC) Wiens & Polhil And Hyphaene Thebaica (L.) Mart

Abstract

Thechlomfimn, methanolandaqlmolsexhactsofflxeleaffibexs ofliyphaene thebaica (Fam_Pahmle) and the leaves of Plioaupalus auzciae @ e) were screened for theirantimiembialactivityusingthempplateagardiflilsionmethod. Theywexetmtedagainst five s1andardbacte:i1"two(izalnpositive(BrzcilIus.s-ubzilrlr, Szaph_yIo0accusazzrezzs),tlneeG1am negative culi, Proteus vulgariv, Pseudomonas aeruginosn) and two slzmdzni fimgi The leaf fibexs chlomfonn em-ant of H.thebaiaz showed moderate activity (1Smm) against the Gmm positive bacteria (B.snbtilis-) and the Gram negative bacteria (Psaemginosa) and‘ one fimgusflalbicansand inactiveaginstflxexestotganismstrsteiltsmeflnmol exhactexerted high activity (17-19 mm)against both Gram positive bacteria, pronounced activity (24|nm) against E0011‘ and moderate activity (l5mm) aginst Pr.wIgan's,Ps,aerugi:zo.su and also high activity (l7mm) againstthetwo fungi ,A.nigerandC.aE5icam Theaqueousextactofthelezffibasof lithebaiva exbfl>iledmodexal:eaefivity(l5mm)against B.subtilis and high activity (I7-18mm) againstE.ooliandCafiiam.\' and inactive against the nest organisms tested. The leaves chlomfinm extract of Plioosepalus amcine showed high activity (16mm) against Ecoli and Il'l0d€l81‘B activity (l5mm) against Psaemgfizaw and the fimgus C.aHn'can.\'.Iis mahmolexuadexhfiwdhighmfifityagakstaflotganisnstmwdlfieaqmomexmmof P.amciae exerted activity (20,16,l7mm) Bsublilir ,S.aurem‘, Ecoli and moderate activity (14mm)againstPl:WlgHfiS. Ps.aemg1'msaand1hefimgusA:zigerandinacfiveagainst Caflzimns. Theminhnmnfi1m"bimxyumomuafiom(MICs)ofthemostaofivemelhmole2macsof H}phameflwbmbamndPHwxepabuamaheagah1st1hesmndaldbadmiawmedmemined usingtheagezplaiedilmionmefixod. The antibactmialandamifimngalaetivifis againstseveurefinmeednlgs weledetetminedagainst the fivemsted GxmnposifivemdGxmnuegfivebaeteliaandfllemntestedfimgiandcompmed- toflaeanfizaxderialandanfifimgalaciivityofthetesmedpiarnmdxacts.

Themostactiveactracls againstthesiandardbacteiiawereselectedandwexethmmsiedagainst 100 clinical isolates collected randomly fiom the National Health laboratory, Faedail Madical Centar and Omdmman Teaching Hospital In this study, the wmmd healing efiiact of Plicosepalus aoaciae leaves extant was invstigated on opm skin wmmd model on thirty Swis Wisiar Albino rats. The active methanol extract ofPIicas~epalus acacia: leaves was prepared in polyefliylene glycol .Fucidin ointment 5% was used as standard 0ontmL The ointmmts were ayplied twice daily on the experimental wounded rats. The results showed that the healing of methanol eximot of Plicosepalus acacia: ointment is Easter than the Fucidin.