Isolation of Some potentially Pathogenic Bacteria From the Upper Respiratory System Khartoum Hospital Staff and patients

Abstract

Abstract ‘ 100 sample from throat and nasal cavity were cultured for aerobic bacteria. Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus viridans, E.coli and Klebsiella pneumoniae were isolated from nasal cavities and throat specimens of healthy medical stall, patients and eo- patients who were not suffering from symptoms ol’ respiratory disorders;the presence~=of these organisms indicated acquired and nosocomial transmission within hospital environment as those are not normal microbiota ol’ the throat. Isolation of pure -isolates as 56.9% from volunteers and 43.1% were mixed isolates .'l‘he aerobic growths were idcntilicd as Gram- positive bacteria which were l‘urther identified as Stupl1ylococcus aurcus (21.1%), Staphylococcus cpiu'ermidi.s' (36.8%), Staphylococcus saprophyticus( l 4.0%), Streptococcus pneumo/1iac(5 3%), Streptococcus viridatts (19.3%) and Corynebactcrium spp (3.5%). Gram-negative bacteria were identified as ’E.c0li (42.9%) and Klebsiclla pncuntoniue (57.1%). Nasal specimens revealed isolation 01' Staphylococcus aurcus, I5. colt, Klebsiella pncumoniae and Pscudomonas aeruginosa which are not among normal llora of the nasal cavitis and considered as potential pathogens. The aerobic growths were

identilied as Graln- positive bacteria which were further identified as Staphylococcus aureus (20.6%), Staphylococcus epidermidis(55.5%), Streptococcus pneum0niae(7.9%), Streptococcus viria'zm.5' (7.9%), Other /J’-hacmolylic s//'epI1__1c0cci (3.3%) and Corynebaclerium spp (4.8%). Gram-negative bacteria were identified as E.c0li (14.3%), Klebsie/la pnezlmoniae (42.9%), I’.s'cud0/no/1u.v zle!'z/gilwsa (28.5%) and Neisseria spp(l4.3%).ls0lated bacteria showed markedly low inhibition zones towards antibiotics . Staphylococcus aureus in all test antibiotics showed resistance and inhibition zone ranged between 3-l lmm, pencillin gave a small inhibition zone. Iicoli showed resistance towards Clindamyein and amoxicillin. I’seuu'0mona.s' ucrugi/10.s'u showed resistance toward nitrofloxacin ,clindainyein and amoxicillin while Klcbsiella pneumoniae was resistant to nitrofloxicin , clindamycin, amoxicillin and tetracycline