Antimicrobial Activity of T rach yspermum ammi Against Some Clinical Isolates

Abstract

Abstract This study was carried out in Khartoum Teaching l-lospital and » Department of Microbiology and Molecular Biology, Faculty ol‘Scieuee and Technology, EL Neelain University. Evaluation of possible antimicrobial activity of Tmchyspermum ammi, also known as (Cnrum cupticmn, Carum ajowan, Traclzyspermum copticum) seeds extracts in ditlerent concentrations against Slap/1yI0c0ccu.r aurcus, l£.co/i, 1’/'0!cu.\' WI/).{(II'I.\‘, 1’scua'0m0na.s' aeruginosa and Candida aII)icun.\' was made. 'l‘hese-orgauistus were isolated from dil‘t'erent specimens (ear and eye swabs, urine, wounds). Out of -l3_4 collected specimens, .S'Ia/J/1yl0cr)ccu.\' auruu.s' represented a high incidence 59 (44%), whereas (fund/'1/u ullriculm showed lower incidence 9 (7%) ~ The plant seeds were extracted successively using petroleum ether, ehlor0l'orm, ethanol and water extracts, and the extracts were tested l'or activity against isolated microorganistns. It was observed that petroleum ether and ethanol extracts t'rom the plant seeds were active; the aqueous and clilo|'oloriu extracts were, however, less active.'This might be due to the presence and concentration ol' active constituents more extractable with petroleum ether and ethanol than water or chlorol'orm. The antimicrobial activity ol' the plant extracts were compared with the activity ofthc antibiotic gentamiein. ' ' Phytochemical analysis ot' the plant seeds demonstrated the presence ot alkaloids, flavone aglyeones and coumarins in petroleum ether extract, anthraquinones, coumarins, anthrasine glycosides and condensed tannins in alcohol fraction and triterpene glycosides, saponins, alkaloids and condensed tannins in aqueous extract.