PHYTOCHEMICAL, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITES OF BOSCIA SENEGALENSIS SEED

Abstract

Abstract This study was conducted with the aim to extract and analyze the seeds powder of Boscia senegalensis , which collected from forest on the north of Alfasher in Darfour state, Sudan. Two methods of extractions from the seeds powder of B.seneglensis were performed ;method 1: extraction by maceration using three different solvents including methanol, water and ethyl acetate their yields were 29% ,9.6% and 17.3% , respectively. These extracts were subjected to phytochemical analysis which showed the presence of saponins, coumarins, carbohydrates, tannins, flavonoids and alkaloids. Method 2: successive extractions using soxhlet extractor from seeds powder using n- hexane, chloroform and methanol yielded 4.7%, 4.1% and 15.7 %, respectively. GC-MS was used for quality and quantity evaluation of the oil extract of seeds revealed the presence of a total of 14 compounds were completely identified and the major compounds were classified as unsaturated fatty acid including Tetratiacontane (22.71%),. Hexaatriacontare (18.41%),Linolenic acid(16.75%) Hexadecanoic acid(11.85),Oleic acid (7.14% ) and Squalene(5.16%) . Anti oxidant activity was evaluated for all extracts. The results showed that the methanol extract (mac) possessed the highest activity with 76.7% at the concentration of 0.1mg/ml using DPPH assay. The most active extract was subjected to TLC and visualized with vanillin –H2SO4 reagent, afforded nine pure analyzed by spectroscopic methods (Infra-red and Ultra-Violt ).