Generation and immune-characterization of single chain fragment variable (scFv) antibody recognize breast cancer cells line (MCF-7)
| dc.contributor.author | Ahmed K Bolad, Ilham Mahgoub | |
| dc.contributor.author | Mohamed Mergani | |
| dc.date.accessioned | 2017-09-27T06:55:34Z | |
| dc.date.available | 2017-09-27T06:55:34Z | |
| dc.date.issued | 2014-02-01 | |
| dc.description.abstract | Phage display technologies were used to produce singlechain antibodies (scFv) against breast cancer cell line (MCF-7). The mouse B cell hybridoma line C3A8 was the starting material, which generates a monoclonal antibody against breast cancer cells (MCF-7). The best candidate scFv sequences, based on (ELISA) screening data were sub-cloned into HB2151 host strain. The scFv gene was expressed in E. coli cytoplasm for further analysis. The purified scFv protein was characterized using western blot, flow Cytometery, indirect ... | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/5547 | |
| dc.language.iso | en | en_US |
| dc.publisher | BioMed Central | en_US |
| dc.relation.ispartofseries | Volume 2;Pages 6 | |
| dc.subject | Breast cancer cells | en_US |
| dc.subject | Antibody recognize | en_US |
| dc.title | Generation and immune-characterization of single chain fragment variable (scFv) antibody recognize breast cancer cells line (MCF-7) | en_US |