Generation and immune-characterization of single chain fragment variable (scFv) antibody recognize breast cancer cells line (MCF-7)

Abstract

Phage display technologies were used to produce singlechain antibodies (scFv) against

breast cancer cell line (MCF-7). The mouse B cell hybridoma line C3A8 was the starting

material, which generates a monoclonal antibody against breast cancer cells (MCF-7). The

best candidate scFv sequences, based on (ELISA) screening data were sub-cloned into

HB2151 host strain. The scFv gene was expressed in E. coli cytoplasm for further analysis.

The purified scFv protein was characterized using western blot, flow Cytometery, indirect ...