College of Graduate Studles " Molecular and conventional studies on Chlamydia trachomatis infection among Gynecological Patients attending ' Khartoum Teaching Hospital

Abstract

ABSTRACT Objective To detect Chlamydia trachomatis among gynecological patients attending Khartoum Teaching Hospital. Materials and methods About l5-20ml first catch urine was obtained from each woman and transported to the laboratory for microscpoical examination. Endocervical smears were collected aseptically from cervix using speculum and one spatula for C. trachomatis PCR test. Another spatula of cervical smear was taken for cytology examination. The speciments were collected in the period Feb, 2007 - Feb, 2011. Ethical consideration was obtained from the patien adminstration in Khartoum Teaching Hospital. Results A total of 191 out of 200 samples were with vaginal discharge and 43 (22.5%) of them were found positive for Chlamydia trachomatis by PCR test, 32 (17.5%) were found positive for Chlamydia trachomatis by Giemsa stain, and 9 (0.1%) were found positive for C. trachomatis by cytology examination. Most positive cases were in the age range is 26- 40 years, where negative cases were in the age range 61 years and above Conclusion - The age range 20-40 years is considered a risk factor that exposes sexually —active women to Chlamydia trachomatis infection. Chlamydia trachomatis infection is becoming a public health problem in Khartoum due to its highest frequency among fertile and non-fertile Sudanese women.