Genetic polymorphisms of catalase and superoxide dismutase in Sudanese patients with vitiligo. A case – control study.

Abstract

Abstract Background:Vitiligo is the most common pigmentary skin disorder. It is a multifactorial polygenic diseaseof unknown aetiology, with epidermal melanocytes destruction.The role of the reactive oxygen species and antioxidant enzymes in its pathogenesis was not fully elucidated. In addition, the disease was not extensively studied at the genes level. Objective:We sought to to investigate the genotype variations in the CAT-21A/T SNP in the catalase (CAT) gene and in the SOD1-251A/G SNP in the superoxide dismutase1 (SOD1) gene, betweenvitiligo patients and an age – and sex – matched control group. Methods:DNA was extracted from the whole blood of 54 Sudanese vitiliginous patients and 55 matched control individuals by the spin column procedure.The CAT and SOD1 genes were amplified by the polymerase chain reaction (PCR); and the SNPs were genotyped by the restriction fragment length polymorphism (RFLP) technique using HinfI and MspI restriction enzymes. Results:No significant difference in the alleles’frequencies of the CAT-21A/T and SOD1-251A/G SNPs had been detected between the vitiligo patients and the control group. Moreover, no allele or genotype polymorphism in these two SNPs can be regarded as a risk or a protecting factor for vitiligo. Conclusion:The CAT-21A/T and SOD1-251A/G SNPs have no association with the pathogenesis of vitiligo.