Al NEELAIN JOURNAL OF GEOSCIENCES - VOL : 01 - ISSUE - 01

Permanent URI for this collectionhttps://repository.neelain.edu.sd/handle/123456789/11373

Browse

Filters

2010 - 20192

Settings

End date: 2019Subject: search.filters.subject.Medical Laboratory

Search Results

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Assessment of Serum levels of Calcium, Phosphorous and Zinc Levels Amange Sudanese Cigarette Smokers in Gazira State
    (Al-Neelain University, 2019-06) Alawad Alwasila Ahmad Ali
    Abstract Background: Across sectional study conducted in Khartoum state from October 2018 to May 2019. Aim to evaluate the effect of cigarette smoking on calcium, phosphorous and zinc level in Sudanese smokers. Materials and Methods: This study 100 subject were chosen for determination of plasma calcium, phosphorous and zinc level,50 were smokers and 50 apparently healthy non smoker servers as control group for compression. The plasma calcium and phosphorous were estimated by spectrophotometer while zinc was estimated by atomic absorption. Results: Analysis showed that three is significantly decrease in mean value of serum calcium in smokers when compared with control group with p value 0.000. There was significantly negative correlation between calcium level and smoking duration with ( P value 0.028 R value -0.310). Also the study showed phosphorous result was normal in smoker when compared with non smokers group with (p value0.992). Also the study showed significantly decrease on serum zinc level in smoker when compared to nonsmoker group with (p value 0.000) there was significantly negative correlation between zinc level on smokers and duration with (p value 0.000, R value -0.322) Conclusion: According to the result of this study the serum calcium and zinc were significantly decrease on cigarette smoker when compared to nonsmoker while serum phosphorous was insignificantly change on cigarette smoker when compared to nonsmokers. ‪
  • No Thumbnail Available
    Item
    Detection of Venous Invasion in Colorectal Carcinoma Using Different Histological Staining Methods A research submitted as partial fulfillment for the requirement of M.sc. Degree in Medical Laboratory Sciences (Histopathology and Cytology)
    (Al-Neelain University, 2010) Amel Khalaf Allah Elhag Fadlalmula
    Abstract This study was conducted at Neelain University, Histopathology and Cytology Department and Ibn Sina Hospital during period from 2008 to May 2010. The aim of this study is to evaluate whether an elastic fiber stain significantly increases the detection of vascular invasion compared with routinely stained sections in colorectal cancer specimens. In this study fifty four colorectal cancer samples (blocks) were collected from archive of Ibn Sina Hospital include males and females in different age. Blocks of tissue were re-embedded in paraffin wax; two serial sections of 4µm thickness were cut from each block. One section was stained with Hematoxylin & Eosin; the other was stained with Verhoeff van Gieson stain. Tissues were evaluated with investigator and results were confirmed with shown variation by Histopathologist and medical laboratory. The present findings imply that serial sections of tumor blocks in colorectal cancer should be stained routinely by Hematoxylin & Eosin and an elastic stain to ensure an acceptable level of sensitivity in the detection of venous invasion. The use of Elastic stained serial sections increased the detection of venous invasion in colorectal carcinomas compared with Hematoxylin & Eosin only. ملخص البحث أجريت هذه الدراسة في جامعة النيلين ، قسم الانسجة و الخلايا المريضة وإدارة مستشفى ابن سينا خلال الفترة من 2008 إلى مايو 2010. والهدف من هذه الدراسة لتقييم ما إذا كان صبغة الألياف المرنة (الفيرهوف فان جيسون) تؤدي الي زيادة كبيرة في الكشف عن غزو الأوعية الدموية مقارنة مع الصبغ الروتيني الهيمتوكسلين والايوسين في عينات سرطان القولون والمستقيم. في هذه الدراسة تم جمع أربعة وخمسين عينة (بلوك) من سرطان القولون والمستقيم من أرشيف مستشفى ابن سينا وتشمل الذكور والإناث في مختلف الأعمار. أعيد انغماس البلوكات في شمع البارافين. بعد احضارها من الأرشيف ، اخذنا من كل عينة مقطعين علي كل شريحة بسمك 4 ميكرومتر . أحد المقطع صبغ بصبغة الهيمتوكسلين والايوسين فقط ، والآخر صبغ بصبغة الفيرهوف فان جيسون، وتم تحليل المواد النسيجية بواسطة اختصاصي الهيستوباثولوجي. النتائج الحالية تعني أن المقاطع من الورم في سرطان القولون والمستقيم ينبغي ان تصبغ بشكل روتيني بصبغة الهيمتوكسلين والايوسين وبصبغة الفيرهوف فان جيسون، وذلك لتأكيد و ضمان مستوى مقبول من حساسية في الكشف عن الغزو وريدي. وايضاعند استخدام صبغة الألياف المرنة (الفيرهوف فان جيسون) في المقاطع المأخوذة من العينات تزيد من اكتشاف الغزو الوريدي مقارنة مع صبغة الهيمتوكسلين والايوسين فقط.