المقالات العلمية – كلية الطب

Permanent URI for this collectionhttps://repository.neelain.edu.sd/handle/123456789/4594

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Has files: YesSubject: search.filters.subject.Helicobacter pylori

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    Clarithromycin-Resistant Helicobacter Pylori Strains among Dyspeptic Patients in Sudan
    (Al-Neelain Medical Research Centre, 2012) Ahmed Bolad, Nazar Abdalazeem; Hassan Abdul-Aziz, Adam Ahmed Adam; Waleed Hussein Omer
    Introduction: The study aimed at characterizing the mutations in 23S rRNA gene related to Clarithromycin-resistant Helicobacter pylori strains among dyspeptic patients in Khartoum State. Methods: Two hundred gastric biopsies were obtained by endoscopy from 200 patients with dyspepsia. DNA was extracted from culture isolated and relevant mutations in 23S rRNA gene were detected. Results: Out of the 200 biopsies, H. pylori was isolated from 48 (24%) biopsies. Twelve of them were found to be resistant to Clarithromycin. Eight of the resistant strains had both A2143G and A2142G by using restriction enzymes Bsa1and Bbs1. Sequencing the remaining four isolates by PCR detected A2140G mutation. Conclusions: In conclusion, Clarithromycin-resistant H. pylori in Sudan may be the main cause of treatment failure aiming at eradication of the bacterium from patients. Such a finding may necessitate the need for other treatment regimens. More collaborated research in this field is needed.
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    Detection of Helicobacter pylori Reactive IgA and IgG Antibodies Using Enzyme Linked Immunsorbent Assay (ELISA)
    (Sudanese Association of Dermatologists, 2010) Ahmed Bolad, Samah Seif Eldein; Mohamed Lutfi
    Background: Helicobacter pylori (H. pylori) infection is usually acquiredduring the early years of life and persists for several years. Recent investigations pointed to a potential role of H. pylori infection of the upper gastrointestinal tract as a possible causative agent in chronic urticaria (CU).Objectives: This study is aiming at evaluating the relationship between H. pylori and chronic urticaria in Sudanese patients.Methods and patients: Fifty patients with idiopathic CU, twenty five ofother skin disorder and ten healthy controls were enrolled estimated for serum H.pylori Immunoglobulin G (IgG) and A (IgA) antibodies levels using Enzyme Linked Immunsorbent Assay (ELISA). All the results were analyzed using the Microsoft Office Excel (Microsoft Office Excel for windows; 2003) and SPSS (SPSS for windows 17).Results and discussion: The IgG and IgA levels of control group weresignificantly lower compared with both patients with chronic urticaria (P = 0.001 for IgG) (P = 0.001 for IgA) and patients with other skin disorders (P = 0.011 for IgG) (P = 0.006 for IgA). Receiver Operating Characteristic curve (ROC curve) analysis revealed that the area under ROC curves of IgA is higher compared to IgG (0.80 0Vs 0.890). In conclusion the current study revealed that patients with H. pylori infection have an increased tendency to develop urticaria. H. pylori reactive IgA antibodies correlate best with the infection.
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    Comparison of different diagnostic methods of Helicobacter pylori infection in Sudanese patients
    (Alneelain University, 2012) Ahmed Kamal Bolad, Nazar Abdalazeem Osman Abdalsadeg; Adam Ahmed Adam, Hassan Abdul-Aziz; Waleed Hussein Omer, Hassan Abdalsalam Osman
    This study aimed at comparing a PCR method of direct detection from biopsy and two other methods: culture and (campylobacter like organism) CLO test for the diagnosis of H. pylori in Sudan. A total of 100 biopsies were taken from 100 patients presenting with various gastroduodenal symptoms after obtaining an informed consent. The biopsies were analyzed using the culture method, CLO test kit and the PCR test using the 23S rRNA gene (Jene bioscience kits). With culture 48% (48 out of 100) were positive, CLO test 53% (53 of 100) were positive, 58 out of 100(58%) were patients by using PCR. Sensitivity and specificity of culture technique was (78%) and (94%) respectively while PCR showed a sensitivity of 94% and a specificity of 75% and CLO test showed (96% & 86.5%) for sensitivity and specificity when culture was the golden method and (88%, 95%) when PCR was the golden method. The PCR test appears to be the most reliable test for diagnosis of H. pylori in Sudan especially where culture is difficult.