Clarithromycin-Resistant Helicobacter Pylori Strains among Dyspeptic Patients in Sudan

Abstract

Introduction: The study aimed at characterizing the mutations in 23S rRNA gene related to

Clarithromycin-resistant Helicobacter pylori strains among dyspeptic patients in Khartoum State.

Methods: Two hundred gastric biopsies were obtained by endoscopy from 200 patients with

dyspepsia. DNA was extracted from culture isolated and relevant mutations in 23S rRNA gene

were detected.

Results: Out of the 200 biopsies, H. pylori was isolated from 48 (24%) biopsies. Twelve of them

were found to be resistant to Clarithromycin. Eight of the resistant strains had both A2143G and

A2142G by using restriction enzymes Bsa1and Bbs1. Sequencing the remaining four isolates by

PCR detected A2140G mutation.

Conclusions: In conclusion, Clarithromycin-resistant H. pylori in Sudan may be the main cause

of treatment failure aiming at eradication of the bacterium from patients. Such a finding may

necessitate the need for other treatment regimens. More collaborated research in this field is

needed.