Browsing by Author "Waleed Hussein Omer"

Filter results by typing the first few letters
Now showing 1 - 4 of 4
  • Thumbnail Image
    Item
    Clarithromycin-Resistant Helicobacter Pylori Strains among Dyspeptic Patients in Sudan
    (Al-Neelain Medical Research Centre, 2012) Ahmed Bolad, Nazar Abdalazeem; Hassan Abdul-Aziz, Adam Ahmed Adam; Waleed Hussein Omer
    Introduction: The study aimed at characterizing the mutations in 23S rRNA gene related to Clarithromycin-resistant Helicobacter pylori strains among dyspeptic patients in Khartoum State. Methods: Two hundred gastric biopsies were obtained by endoscopy from 200 patients with dyspepsia. DNA was extracted from culture isolated and relevant mutations in 23S rRNA gene were detected. Results: Out of the 200 biopsies, H. pylori was isolated from 48 (24%) biopsies. Twelve of them were found to be resistant to Clarithromycin. Eight of the resistant strains had both A2143G and A2142G by using restriction enzymes Bsa1and Bbs1. Sequencing the remaining four isolates by PCR detected A2140G mutation. Conclusions: In conclusion, Clarithromycin-resistant H. pylori in Sudan may be the main cause of treatment failure aiming at eradication of the bacterium from patients. Such a finding may necessitate the need for other treatment regimens. More collaborated research in this field is needed.
  • Thumbnail Image
    Item
    Clarithromycin-Resistant Helicobacter Pylori Strains among Dyspeptic Patients in Sudan
    (جامعة النيلين- مركز النيلين الطبي, 2012) Nazar Abdalazeem; Hassan Abdul-Aziz; Adam Ahmed Adam; Waleed Hussein Omer; Ahmed Bolad
  • Thumbnail Image
    Item
    A clinical study of serum calcium, phosphorus, and alkaline phosphates level in type II Diabetes mellitus among Sudanese population in Khartoum State, 2012
    (جامعة النيلين- مركز النيلين الطبي, 2013) Nasir Abdelrafie Hamad1; Linda Bashier Eltayeb; Sara Lavinia Brair; Khalid Hussein Bakheit; Hamdan Zaki Hamdan; Waleed Hussein Omer
  • Thumbnail Image
    Item
    Comparison of different diagnostic methods of Helicobacter pylori infection in Sudanese patients
    (جامعة النيلين, 2012) Nazar Abdalazeem Osman Abdalsadeg; Adam Ahmed Adam; Hassan Abdul-Aziz; Waleed Hussein Omer; Hassan Abdalsalam Osman; Ahmed Kamal Bolad
    This study aimed at comparing a PCR method of direct detection from biopsy and two other methods: culture and (campylobacter like organism) CLO test for the diagnosis of H. pylori in Sudan. A total of 100 biopsies were taken from 100 patients presenting with various gastro-duodenal symptoms after obtaining an informed consent. The biopsies were analyzed using the culture method, CLO test kit and the PCR test using the 23S rRNA gene (Jene bioscience kits). With culture 48% (48 out of 100) were positive, CLO test 53% (53 of 100) were positive, 58 out of 100(58%) were patients by using PCR. Sensitivity and specificity of culture technique was (78%) and (94%) respectively while PCR showed a sensitivity of 94% and a specificity of 75% and CLO test showed (96% & 86.5%) for sensitivity and specificity when culture was the golden method and (88%, 95%) when PCR was the golden method. The PCR test appears to be the most reliable test for diagnosis of H. pylori in Sudan especially where culture is difficult. Keywords: Helicobacter pylori, biopsies, PCR, CLO test, culture.