Browsing by Author "Ahmed K Bolad, Ilham Mahgoub"

Filter results by typing the first few letters
Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    Generation and immune-characterization of single chain fragment variable (scFv) antibody recognize breast cancer cells line (MCF-7)
    (BioMed Central, 2014-02-01) Ahmed K Bolad, Ilham Mahgoub; Mohamed Mergani
    Phage display technologies were used to produce singlechain antibodies (scFv) against breast cancer cell line (MCF-7). The mouse B cell hybridoma line C3A8 was the starting material, which generates a monoclonal antibody against breast cancer cells (MCF-7). The best candidate scFv sequences, based on (ELISA) screening data were sub-cloned into HB2151 host strain. The scFv gene was expressed in E. coli cytoplasm for further analysis. The purified scFv protein was characterized using western blot, flow Cytometery, indirect ...