Mohammed Nafi Hammad Manzool2018-10-152018-10-152006http://hdl.handle.net/123456789/13215M.Sc. degree in medical microbiologyChlamydia trachomatis infections are now recognized as the leading cause of sexually transmitted diseases (STD). It is known to be subject to cervicitis, pelvic inflammatory disease (PID), infant conjunctivitis, infant pneumonia and urethritis. If left untreated properly they can lead to severe squeal in women such as pelvic inflammatory disease, ectopic pregnancy, and tubal infertility. Babies born to chlamydia infected mothers are at high risk of developing inclusion conjunctivitis and pneumonia. Several methods are available for the detection of C. trachomatis in clinical specimens, such as Giemsa staining, cell culture, direct antigen detection, and nucleic acid probe. Recently polymerase chain reaction (PCR) technology has been focusing on the diagnosis of infectious diseases. It can also provide a rapid, convenient and sensitive detection of C. trachomatis in clinical specimens. The aim of this study was to estimate the prevalence of Chlamydia trachomatis infections, among pregnant women, using polymerase chain reaction, during the period of 2004 and 2006, to asses the pattern of chlamydia infections. Atotal number of one hundred and fifty two pregnant women attending Omdunnan Maternally hospital, were involved in this study. The study group had an age average between l6 and 43 years with a mean of 27.9 years. About 10.5% of the study group were in the first trimester, while 32.6% and 57.9% were in the second and third trimester respectively. The frequency of Chlamydia trachomatis infections, using polymerase chain reaction, was 16.4% (25/ 152) among studied group. Previous history of abortion was detected in 43.4% of study population, 52% of them have Chlamydia trachamatis infections. Frequency of polyuria was detected in 74.3% of study population, 84% of them have Chlamydia trachomalis infections. Dysuria was detected in 7.2 % of study population, 16% of them have Chlamydia t/‘ac/zoznaris infections. Pyuria was detected in 78.9% of study population, 80% of them have Chlamydia trachomatis infectionsenmedical microbiologyThesis