Ahmed K Bolad, Ilham MahgoubMohamed Mergani2017-09-272017-09-272014-02-01http://hdl.handle.net/123456789/5547Phage display technologies were used to produce singlechain antibodies (scFv) against breast cancer cell line (MCF-7). The mouse B cell hybridoma line C3A8 was the starting material, which generates a monoclonal antibody against breast cancer cells (MCF-7). The best candidate scFv sequences, based on (ELISA) screening data were sub-cloned into HB2151 host strain. The scFv gene was expressed in E. coli cytoplasm for further analysis. The purified scFv protein was characterized using western blot, flow Cytometery, indirect ...enBreast cancer cellsAntibody recognize