Induced Breeding of Clarias lazera and Labeo il t' using Chicken Pituitary Gland, HCG and ll'l 0 ICUS SGnRHa

Abstract

ABSTRACT In this study is used hormones of Chicken PG ,HCG and SGnRHa then it mixed this hormones with PG to induce fish of Claries lazera and Labeo niloticus . It gave best results in inducing of this fish. It used one, two, and three gland/kg/fish body, it found 3 glands / kg fish body weight was best dose it gave (56.5 % success in Laggg females, 47.6% in niloticus females). It found mixing of PG , HCG and SGnRHa was best and effective is increased Doses of 1000, 1500, 2250, and 2550 IU HCG alone / kg female lggeg were 100, l00, 100 and 66.6 % successful. Doses of 900, 2250 and 5000 IU per kg female were 40, 60 and 80 % successful. All doses used with males of both fishes were not successful. 0.3, 0.4 and 0.5 ml SGnRI-Ia per kg female E and were 62.5 — 87.5 % successful for lggra and 50 — 83.3 % successful for in all successful striping trials with female the latency period ranged between 8 —I 15 hours. All doses of chicken PGE and hormones used with males of both fishes were not successful. To fertilize stripped eggs semen obtained from two sacrificed males (two from each species) was used successfully to fertilize eggs stripped from one fish of the same species. Fertilized eggs were reared in Neelain University Farm Hatchery. Hatching of eggs occurred after 24 - 48 hours at 25 - 33C“. Survival, counts were carried 5 days after incubation. Survival of hatched larvae ranged between l and 60 %. IV