كلية علوم المختبرات الطبية
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Item Frequency of Schistosoma haematobium and Salmonella Co-infection in Khartoum-state, Sudan(Al-Neelain University, 2022-07) Walaa Mohamed NuryAbstract Background Schistosomiasis is a neglected tropical disease and it remains the second most afflicting parasitic illness especially in sub-Saharan African countries. The interaction between schistosomiasis and Salmonella is a particularly important issue in Africa, where dual infection by the parasite and the bacterium are likely common. this study aimed to detect of Schistosoma haematobium and Salmonella Co-infection in Khartoum-state, Sudan. Methods The present study was cross sectional study carried out during February to July 2022 in Khartoum State. Total of one hundred Schistosoma haematobium infected patients were enrolled in this study. Urine examination were performed to confirm the presence of Schistosoma haematobium, anti-Salmonella typhi antibodies in patients serum were detected using Immunochromatographic test. Findings Out of one hundred Schistosoma haematobium infected patients examined in this study, 52%were positive result by ICT Salmonella test. Conclusions There was high frequency of anti-Salmonella antibodies among Schistosoma haematobium infected patients. In conclusion, I recommend that all Shistosomiasis patient get tested to detect presence of Salmonella, because most of the symptoms can be caused by salmonella الخلاصة الخلفية داء البلهارسيا هو مرض استوائي مهمل ويظل ثاني أكثر الأمراض الطفيلية إصابة خاصة في البلدان الأفريقية جنوب الصحراء الكبرى. يعتبر التفاعل بين داء البلهارسيا والسالمونيلا قضية مهمة بشكل خاص في إفريقيا ، حيث من المحتمل أن تكون العدوى المزدوجة من الطفيلي والبكتيريا شائعة ، وتهدف هذه الدراسة إلى الكشف عن عدوى البلهارسيا الدموية وعدوى السالمونيلا المشتركة في ولاية الخرطوم ، السودان. الطرق: الدراسة الحالية عبارة عن دراسة مقطعية أجريت خلال شهر فبراير إلى يوليو 2022 في ولاية الخرطوم. تم تسجيل مجموعه مائة مريض مصاب بالبلهارسيا الدموية في هذه الدراسة. تم إجراء فحص البول للتأكد من وجود البلهارسيا الدموية ، تم الكشف عن الأجسام المضادة للسالمونيلا في مصل المرضى باستخدام اختبار الكروماتوغرافيا المناعية (ICT) .. النتائج من بين مائة مريض مصاب بالبلهارسيا الدموي الذين تم فحصهم في هذه الدراسة ، كان 52٪ نتيجة إيجابية لاختبار السالمونيلا الخاص بتقنية (ICT). الخلاصة لقد خلصت هذه الدراسة إلى أن هناك نسبة عالية من الأجسام المضادة للسالمونيلا بين المرضى المصابين بالبلهارسيا. في الختام أوصي بعمل فحوصات شامله للمصابين بداء البلهارسيا لاستبعاد وجود عدوى أخرى لأن معظم الأعراض يمكن أن تكون بسبب السالمونيلا .Item Isolation, Identification and Molecular Characterization of Salmonella Species From different sources(Al Neelain University, 2009-02) Abbas Hassan MohammedA total number of 733 samples were collected fiom different sources (clinical, food, poultry and water), and subjected to bacteriological examination for isolation of Salmonella spp. using enrichment and selective media. The percentages of the detection of the organism from the above sources were 10.5, 2.9,‘ 2.5 and 0.0 respectively. y From the 200 feacal specimens (100 from human and 100 from animals),'the organism was detected in 20% of the human specimens and only as 1% from the animal specimens. Food specimens revealed isolation of Salmonella spp as 10% and 1.85% from meat and milk specimens respectively, while percentages of 5.1 and 1.6 were obtained from chicken and eggs specimens and the raw shaworma revealed negative growth of the Salmonella spp. i Using the API method for confinnation of the isolates gave unacceptable profiles with 66.7%, 44.4% and 66.7% for Salmonella spp isolated from clinical, food and poultry specimens respectively, (in all unacceptable profile organisms the Salmonella spp was the first suggested organism). 41.7% of the total specimens showed confirmation of the presence of the organism with identity of 99.9%. The API also showed that there are strong similarities in biochemical reactions of the Salmonella spp K. pneum. Ozaenae, E. coli 2, E. coli 1 and Hafnia alvei. The serological investigation revealed the identification of Salmonella anatum from both poultry and food specimens, Salmonella allerton from both poulhy and human specimens and Salmonella enteritidis from both food and human specimens. Salmonella Kentucky, Salmonella albertslunal Salmonella abortus bovis, Salmonella tchad, Salmonella II. Other serotypes were detected in human specimens without detection for the common known pathogenic species of Salmonella typhi and Salmonella paratyphi. Food specimens also revealed the presence of Salmonella okerara, Salmonella Harrisonburg and Salmonella maiduguri. _ l All isolates fiom the different sources showed high sensitivity towards Arnykacin, Ciprofloxacine, Cefoperazone and Netilmicin antibiotics.‘ Most of the isolated Salmonella spp showed clear resistance toward Augmentin antibiotic. There were clear variations in the susceptibility of Salmonella spp toward different antibiotics according to their origin of source. The PCR product analysis indicated the presence of the gene associated with virulence in two of the two Salmonella enteritidis serovars with plasmid profiles containing molecular weights of 26, 424 and 51, 418 kb. The Salmonella enteritidis also showed the presence of virulence gene as single amplification product of 460 bp.