Browsing by Author "Soha Mohammed Yahiya"

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    Study of Methylene Tetra hydro Folate Reductase Enzyme Single nucleotide polymorphism (C667T) (rs180113) and Susceptibility to Diabetic Septic Foot among Diabetic Sudanese Patients
    (Al-Neelain University, 2020-02) Soha Mohammed Yahiya
    Abstract Background: Diabetic foot ulcer is considered as the most serious diabetes complication; contribute significantly to morbidity and mortality of diabetic patients in Sudan. Globally there are proven genetic based studies in diabetic septic foot predisposition, whereas a few studies have been done in Sudan. Objective: This study was intended to estimate the association of (C667T) SNP (rs180113) in the methylene tetrahydro folate reductase (MTHFR) enzyme with the DSF predisposition, and the role of some non-genetic risk factors in diabetic Sudanese population. Method: A cross-sectional case-control study was conducted, 55 were diabetic septic foot (DSF) patients represent the case group and 75 diabetic patients (DM) without septic complication as control. The (C667T) (rs180113) Polymorphism was detected by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) using Hinf I enzyme. Results: The analysis of age among cases and controls showed that 94.4% of cases are at age group more than 40 years while 76.4% of controls are at this age group, with a high significant difference between DSF and DM ( p – value = 0.005, OR = 0.190, CI = 0.688 – 0.053), no gender difference has been observed between DSF and DM (p – value = 0.510), DSF with a family history of diabetes are 66.7% and 63.4% of DM have a family history, with no significant difference observed between DSF and DM . 16.7% of DSF are alcohol consumers while only 4.2% of DM. DSF are at four times increased risk than those who don’t consume alcohol (p – value = 0.02 , OR = 4.600) . High frequency of neuropathy (58%) was observed among DSF compared to DM , and a high significant association of neuropathy with DSF with a three times increased risk of DSF (p – value = 0.001, OR= 3.452). DSF with retinopathy showed a high significant difference compared to DM with retinopathy (p value = 0.006), with a three times increased risk of DSF (OR = 3.527). Concerning the genotype analysis, the homozygous wild type genotype CC showed high frequency among cases and control with the following percentage (96.7% , 100%) respectively. No significant association between the mutant genotype and DSF predisposition. Conclusion: The results of this study indicates that MTHFR (C667T) (rs180113) polymorphism is not associated with the increased risk of diabetic septic foot among Sudanese diabetic patients, and neuropathy, retinopathy might be a strong pathological cases lead to diabetic septic foot development.
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    Study the association of Methylenetetrahydrofolate reductase gene polymorphism (C667T) (rs180113) and Diabetic Septic Foot among Diabetic Sudanese Patients
    (Al-Neelain University, 2020) Soha Mohammed Yahiya
    Abstract Background: Diabetic foot ulcer is considered as a common diabetes complication; contribute significantly to morbidity and mortality of diabetic patients in Sudan. Globally there are proven genetic based studies in diabetic septic foot predisposition, whereas a few studies have been done in Sudan. Objective: This study was intended to estimate the association of (C667T) SNP (rs180113) in the methylene tetrahydro folate reductase (MTHFR) gene with the DSF predisposition, and the role of some non-genetic risk factors in diabetic Sudanese population. Method: Hospital based cross sectional study was conducted, 55 were diabetic septic foot (DSF) patients represent the case group and 75 diabetic patients (DM) without septic complication as control. The (C667T) (rs180113) Polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using Hinf I enzyme. And the other demographic data was analysis using cross tabulation and person correlation test to estimate the p-values and odd ratios by SPSS v21. Results: The statistical analysis of age among cases and controls showed that 94.4% of cases are at age group more than 40 years while 76.4% of controls are at this age group, with a high significant difference between DSF and DM ( p – value = 0.005, OR = 0.190, CI = 0.688 – 0.053), no gender difference has been observed between DSF and DM (p – value = 0.510) with 2:1 male to female ratio , DSF with a family history of diabetes are 66.7% and 63.4% of DM have a family history, with no significant difference observed between DSF and DM. Alcohol consumption is significantly higher in DSF than DM (P-value = 0.02). DSF are at four times increased risk than those who don’t consume alcohol (p – value = 0.02 , OR = 4.600). High frequency of neuropathy (58%) was observed among DSF compared to DM , and a high significant association of neuropathy with DSF with a three times increased risk of DSF (p – value = 0.001, OR= 3.452). DSF with retinopathy showed a high significant difference compared to DM with retinopathy (p value = 0.006), with a three times increased risk of DSF (OR = 3.527). Concerning the genotype analysis, the homozygous wild type genotype CC showed high frequency among cases and control with the following percentage (96.7% , 100%) respectively , the homozygous mutant genotype TT represent 3.30% of DSF and 0.0% of DM, while the heterozygous genotype TC represent 0% of both DSF and DM, no significant association between the mutant genotype and DSF predisposition. Conclusion: The results of this study indicates that MTHFR (C667T) (rs180113) polymorphism is not associated with the increased risk of diabetic septic foot among Sudanese diabetic patients, and neuropathy, retinopathy and diabetic septic foot might be a linked diabetes complication . المستخلص الخلفية: القدم السكرية هي من أخطر مضاعفات مرض السكري ، وتسهم بشكل كبير في وفيات مرضى السكري في السودان عالميا هناك دراسات مثبتة على دور العوامل الوراثيه في ​​مدى الاصابه بمرض القدم السكريه ، في حين أجريت دراسات قليلة في السودان وعلى المستوى العالمي كل 30 ثانيه هناك اعاقه تحدث بسبب السكري (Gershater M. A. et al., 2009 ) الهدف: هذه الدراسة تهدف لقياس ارتباط SNP (C667T) (rs180113) في إنزيم MTHFR مع جروح السكري ودور بعض عوامل الخطوره الغير وراثية في السكان السودانيين المصابين بالسكري. الطريقة: أجريت الدراسه على المصابين بالسكري ذوي القدم السكريه ومرضى السكري الذين لم يصابو بالقدم السكريه ، 55 كانوا من مرضى السكري ذوي القدم السكريه يمثلون الحالات و 75 مريضا بالسكري دون مضاعفات القدم السكريه يمثلون مجموعه التحكم ,تم الكشف عن تعدد اشكال الطفره (C667T) (rs180113) في جين ال mthfrعن طريق استخدام جهاز تفاعل البلمره المتسلسل و تقنيه استخدام انزيمات القطع المتخصصه في تعدد اشكال الطفرات (PCR-RFLP) باستخدام إنزيمHinf I ودور بعض العوامل غير الوراثيه وتحليلها باستخدام برنامج التحليل الاحصائي SPSS باستخدام اختبار بيرسون لايجاد قيمه p. النتائج: أظهر تحليل العمر بين الحالات والضوابط أن 94.4 ٪ من الحالات في الفئة العمرية أكثر من 40 سنة في حين أن 76.4 ٪ من الضوابط في هذه الفئة العمرية ، فرق كبير ملحوظ لوحظ بين DSF و DM فيما يتعلق بالعمر (p - value = 0.005 ، OR = 0.190 ، CI = 0.688 - 0.053) ، لم يلاحظ أي فرق في الجنس بين DSF و DM (ع - القيمة = 0.510) ،وجد ايضا ان الحالات مع تاريخ العائله لمرض السكري هي 66.7 ٪ و 63.4 ٪ من DM واستخلص انه يوجد ارتباط كبير فيما يتعلق بتاريخ العائله P- value = 0.426) ، 16.7 ٪ من مرض السكري DSF هم من مستهلكي الكحول بينما 4.2 ٪ فقط من DM ، لذلك فإن مجموعه التحكم االتي تتعاطى الكحول في خطر أربع مرات من الذين لا يستهلكون المشروبات الكحوليه (p - value = تمثل 0.02 ، OR = 4.600) ، 58٪ من DSF لديهم اعتلال عصبي و 28.6٪ من DM ، يمثلون ارتباطًا مهمًا بينهم ، DM الذين لديهم اعتلال عصبي معرضون لخطر ثلاث مرات أكثر من الذين ليس لديهم (ع - القيمة = 0.001 ، أو = 3.452) ، 35.2 ٪ من DSF لديهم اعتلال الشبكية بينما أظهر 14.3 ٪ من DM ، فرق كبير كبير ب لم يظهر أي من DM و DSF ومرضى السكري الذين يعانون من اعتلال الشبكية في خطر ثلاث مرات أكثر من مرضى السكري دون اعتلال الشبكية (ع - القيمة = 0.006 ، أو = 3.527) ، أمراض الكلى ، ارتفاع ضغط الدم وأمراض القلب لم تظهر فرقاً كبيراً بين DSF و DM (p- القيم: 0.420 ، 0.382 ، و 0.476 على التوالي) ، فيما يتعلق بالتركيب الوراثي ، لم يلاحظ أي فرق كبير بين الحالات (p-value = 0.732). الخلاصة: تشير نتائج هذه الدراسة إلى أن تعدد الأشكال MTHFR (C667T) (rs180113) لا يرتبط بزيادة خطر القدم المصابة بمرض السكري بين مرضى السكري السودانيين ، الاعتلال العصبي و اعتلال الشبكية.قد يكونان مرتبطان بظهور القدم السكريه .