Browsing by Author "Shami Elhaj Alssafi Bakhiet"

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Effect of Some Isolated Actinomycetes Indigenous to Sudanese Soils on Fusarium oxysporum, Aspergillus fumigatus and Candida albicans
(Alneelain University, 2014) Shami Elhaj Alssafi Bakhiet
The test-organisms include two moulds and a yeast-like fungus; Fusarium oxysporum which was obtained from Faculty of Agriculture — University of Khartoum, while Aspergillus fumigatus and Candida albicans were obtained from Quality Control Laboratory of General Medicine Company. All test organisms were examined microscopically to conﬁrm their purity and cultured into Sabouraud dextrose agar medium to test their viability. Thirty samples were collected from the soil of Tuti Island’s farms and the Blue Nile river bank. During this study, twenty ﬁve Actinomycetes species were isolated using Starch Casein agar medium, and characterized by their morphological appearance and by biochemical tests. These isolates, after characterization were found to belong to three genera, namely; Streptomyces (19 species), Actinomyces (5 species) and one species belongs to Arachnia (formerly Actinomyces). All the isolates were screened and evaluated for their antifungal activity against three test organisms using Cross Streak and Modiﬁed Cross Streak methods and incubated for 3 days and 5 days. Sixteen of the isolated Actinomycetes showed antifungal activities against the two moulds at both incubation periods (3 and 5 days). While only six isolates Actinomycetesshowed inhibitory effect against C. albicans. The result showed that F‘. oxysporum has resistance to the inhibitory effect more than A. fumigatus and it has an ability to utilize the secondary metabolites and decrease the intensity of Actinomycetes growth of some isolates and grow over them. The crude ﬁltrates of fermented actinomycetal broth were analyzed by thin layer chromatography (TLC) technique and seventeen different antifungal drugs were characterized according to their Rfvalues.
Isolation and determination of aflatoxin producing agents from stored peanut kernels and peanut butter
(Al Neelain University, 2009) Shami Elhaj Alssafi Bakhiet
اAbstract Sixty samples of each stored peanut kernels and peanut butter were collected from different locations. Stored peanut kernels samples collected from Mayo, Umbaddah cities in Khartoum state, Al-Helalia and Al-Managel cities in Al-Jazeera state. All peanut butters were collected from Khartoum state from four cities (Mayo, Al-Sahafa, Al-Maourada cities and Bahri local market). Fifteen samples of each stored peanut kernels and peanut butter were collected from each cities. All samples were subjected to analysis processes by two methods; microbiological analysis by culturing them into suitable growth medium "Sabouraud's Dextrose Agar" and chemical analysis by Thin-Layer Chromatography (TLC) technique. Thirty five samples (58.33%) from total samples of stored peanut kernels showed aflatoxin B1 chromatographically, while twenty nine samples (48.33%) from the total samples of peanut butter showed positivereading to chromatographical analysis. In culture, Aspergillus flavus was isolated from twenty six samples (43.33%) of stored peanut kernels and eight samples (13.33%) of peanut butter. 10 The concentration of aflatoxin B1 in the test samples were stepping from low, high, and very high, in range of (17.57-404.00 μg/Kg) in stored peanut kernels and in peanut butter is ranging from (13.47-80.80 μg/Kg). الملخص جُمعت ستون عينة من كلاً من حبيبات الفول السوداني المخزون وزبدة الفول السوداني من مناطق مختلفة. وكانت المناطق موزعة على ولايتي الخرطوم (مدينة مايو ومدينة أم بدة) وولاية الجزيرة (مدينة الهلالية ومدينة المناقل) لعينات حبيبات الفول السوداني المخزون. أما عينات زبدة الفول السوداني فقد جُمعت من ولاية الخرطوم (مدينة مايو، مدينة الصحافة، مدينة الموردة وسوق بحري المركزي) بمعدل خمسة عشر عينة لكل مدينة. كل العينات أُختبرت عن طريق التحليل الميكروبي بواسطة التزريع في وسط أجار السابرود ديكستروز وعن طريق التحليل الكيميائي بواسطة تقنية التصوير الملون ذات الطبقة الرقيقة لمعرفة ما إذا كانت العينات ملوثة بسم الافلاتوكسين ب1. خمس وثلاثون عينة بنسبة (%58.33) من مجموع عينات حبيبات الفول السوداني المخزون شوهدت تحتوي على سم الافلاتوكسين ب1 عن طريق تقنية التصوير الملون ذات الطبقة الرقيقة ُووجدت تسع وعشرون عينة بنسبة (%48.33) من مجموع عينات الزبدة الفول السوداني موجبة لإختبار التصوير الملون ذات الطبقة الرقيقة. أما في التحليل الميكروبي فقد عُزل فطر الرشاشية الصفراء من ست وعشرون عينة بنسبة (%43.33) من عينات حبيبات الفول السوداني المخزون ومن ثمان عينات بنسبة (%13.33) من عينات زبدة الفول السوداني. تركيز سم الافلاتوكسين ب1 في العينات المختبرة تراوح مابين منخفض، عالي، وعالي جداً في مدى يتراوح ما بين (17.57-404.00 مايكروجرام/كجم) في عينات حبيبات الفول السوداني المخزون وما بين (13.47-80.80 مايكروجرام/كجم) في عينات زبدة الفول السوداني
Isolation and determination of aﬂatoxin producing-agents from stored peanut kernels and peanut butter
(Neelain University, 2009) Shami Elhaj Alssafi Bakhiet
Abstract Sixty samples of each stored peanut kernels and peanut butter were collected ﬁrom different locations. Stored peanut kernels samples collected from Mayo, Umbaddah cities in Khartoum state, Al-Helalia and Al-Managel cities in Al-Jazeera state. All peanut butters were collected from Khartoum state from four cities (Mayo, Al-Sahafa, Al-Maourada cities and Bahri local market). Fifteen samples of each stored peanut kernels and peanut butter were collected from each cities. All samples were subjected to analysis processes by two methods; microbiological analysis by culturing them into suitable growth medium “Sabouraud‘s Dextrose Agar" and chemical analysis by Thin—Layer Chromatography (TLC) technique. Thirty ﬁve samples (58.33%) from total samples of stored peanut kernels showed aflatoxin B, chromatographically, while twenty nine samples (48.33%) from the total samples of peanut butter showed positive-reading to chromatographical analysis. In culture, Aspergillus ﬂavus was isolated from twenty six samples (43.33%) of stored peanut kernels and eight samples (13.33%) of peanut butter. The concentration of aflatoxin B1 in the test samples were stepping from low, high, and very high, in range of (17.57-404.00 pg/Kg) in stored peanut kernels and in peanut butter is ranging from (13.47-80.80 pg/Kg).