Browsing by Author "Mohammed Abdullah Mohammed Ahmed"

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    College of Graduate Studles " Molecular and conventional studies on Chlamydia trachomatis infection among Gynecological Patients attending ' Khartoum Teaching Hospital
    (Neelain University, 2012) Mohammed Abdullah Mohammed Ahmed
    ABSTRACT Objective To detect Chlamydia trachomatis among gynecological patients attending Khartoum Teaching Hospital. Materials and methods About l5-20ml first catch urine was obtained from each woman and transported to the laboratory for microscpoical examination. Endocervical smears were collected aseptically from cervix using speculum and one spatula for C. trachomatis PCR test. Another spatula of cervical smear was taken for cytology examination. The speciments were collected in the period Feb, 2007 - Feb, 2011. Ethical consideration was obtained from the patien adminstration in Khartoum Teaching Hospital. Results A total of 191 out of 200 samples were with vaginal discharge and 43 (22.5%) of them were found positive for Chlamydia trachomatis by PCR test, 32 (17.5%) were found positive for Chlamydia trachomatis by Giemsa stain, and 9 (0.1%) were found positive for C. trachomatis by cytology examination. Most positive cases were in the age range is 26- 40 years, where negative cases were in the age range 61 years and above Conclusion - The age range 20-40 years is considered a risk factor that exposes sexually —active women to Chlamydia trachomatis infection. Chlamydia trachomatis infection is becoming a public health problem in Khartoum due to its highest frequency among fertile and non-fertile Sudanese women.
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    Molecular and conventional studies on Chlamydia trachomatis infection among Gynecological Patients attending Khartoum Teaching Hospital
    (Al Neelain University, 2012) Mohammed Abdullah Mohammed Ahmed
    ABSTRACT Objective: To evaluate the chemical characterization and antimicrobial activity of different floral origin honey samples against a wide range of bacterial strains , and to verify the nature of the active substance (s) . Methodology: Thirty –two different honey samples collected from different countries (raw and commercial honey ) were tested in vitro for antibacterial activity of standard and clinical isolates using cup plate diffusion technique . The same clinical isolates were tested in vitro for some antibiotics sensitivity. Different honey concentrations, ethyl acetate extracts and water residue were assessed in vitro for antibacterial activity . Topical dressing of cutaneous leishmaniasis of 25 hamsters with natural honey was compared with other 25 group treated by Pentostam injection. Extraction of honey was carried out by petroleum ether and ethyl acetate. The extracts were subjected to thin layer chromatography Ethyl acetate extracts were analyzed by HPLC and GC-MS for chemical Characterization to fingerprint honey samples according to their floral origin . 10 Results: All bacterial strains exhibited marked sensitivity to honey samples, even the most resistant bacterial strains to the commonly used antibiotics –MRSA and Pseudomonas spp. The clinical isolates showed remarkable resistance to the most antibiotics tested. Different honey concentrations inhibited bacterial growth even at low dilution 10%. Ethyl acetate extracts exhibited strong antibacterial activity while it is water residue showed no activity. Honey was found to be an effective treatment for the cutaneous leishmaniasis induced in hamsters as compared with control group which was treated by Pentostam injection. TLC of honey extracst resulted in different terpenoids and phenolic compounds. Analysis of honey samples by using HPLC and GC-MS revealed different phenolic compounds correlated with their botanical and geographical origin . Different honey samples showed different compounds according to their floral origin . Natural and commercial honeys samples exhibited potent antibacterial. Conclusion: The findings of the current study suggests that honey would be beneficial to control resistant bacterial strains without side effects. The active 11 fractions ( antibacterial of honey ) may be a valuable source of future therapeutic. Effectiveness of honey dressing in treatment of leishmanial ulcers gives a promising trend in controlling such cases. GC-MS profile reflect the floral source , and forms a basis of an attempt to fingerprint honey samples . Much of the effectiveness of honey as antibacterial activity was attributed to phenolics flavonoids, some of the volatile compounds , hydrogen peroxide normally present in honey due to the effect of some enzyme, the natural concentration and hygroscopic nature of honey, plus the cidic pH of honey . Honey can be used as a natural product without side effects . 12 مستخلص : الهدف من الأطروحة الأهداف تشخيص ميكروب الكلاميديا بواسطة تحليل الحمض الاميني دي ان اي في المرضي الذين ليس لديهم القدرة علي الانجاب ويترددون علي عيادات العقم في مستشفي الخرطوم التعليمي. المواد وطرق العمل تم جمع حوالي من 15 الي 20 مل من البول في الصباح من المرضي في حاويات نظيفة ومعقمة وذلك لاجراء الفحص المجهري لميكروب الكلاميديا. وايضا تم اخذ عينتان من عنق الرحم من المرضي واحدة لاجراء فحص الحمض الاميني دي ان اي لميكروب الكلاميديا والاخري لفحص الانسجة والخلايا المجهري النتائج تم جمع 200 عينة عشوائية من المرضي الذين يترددون علي عيادات العقم والنساء والتوليد بمستشفي الخرطوم التعليمي حيث تم اخذ عينات من عنق الرحم لاجراء اختباري الحمض الاميني دي ان اي وفحص الانسجة والخلايا للمرضي. وايضا تم جمع 200 عينة بول من المرضي وذلك لاجراء الفحص المجهري. من 191 مريض تم اختبارهم يعانون من الافرازات المهبلية وجد منهم 43 (52.3% ) لديهم نتيجة موجبة لميكروب الكلاميديا عن طريق الفحص الاميني دي ان اي و32(38.1%) لديهم نتيجة موجبة لميكروب الكلاميديا عن طريق الفحص المجهري بصبغة جيمسا و9 (10.7%) لديهم نتيجة موجبة لميكروب الكلاميديا عن طريق فحص الانسجة والخلايا المجهري . ووجد ان الفئة العمرية 26 الي 40 تحتوي علي اكثر النتائج الايجابية اما الفئة العمرية اكثر من 61 تحتوي علي اكثر النتائج السلبية . الاستنتاجات: الفئة العمرية 26 الي 40 تعتبر من اكثر العوامل التي تشير الي نسبة تفشي الاصابة بميكروب الكلاميديا خاصة في النساء . الاصابة بمرض الكلاميديا يؤدي الي مضاعفات خطيرة وهي انسداد انبوب قناة فالوب و الاجهاض. اصبح مرض الكلاميديا من الامراض المنتشرة بصورة كبيرة جدا في الخرطوم نسبة لتفشي المرض في النساء السودانيات الحوامل وايضا اللواتي يعانين من عدم الانجاب