Browsing by Author "Leena Babiker Mirghani"

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Evaluation of Hemoglobin F level and its Association with HaemoglobinA1c among Type 2 Diabetic Patients
(2018) Alsadia Ahmed Hassan Dafalla; Leena Babiker Mirghani
Haemoglobin A1C (HbA1c) level is one of the effective diagnostic tools used in the monitoring of diabetic patients. Furthermore, it can be used as a risk indicator for any development of complications in diabetic patients. There are some evidences that fetal haemoglobin (Hb-F) is also elevated in adults with type 2 diabetes mellitus and children with type1 diabetes mellitus. Elevated Hb-F is suggested to interfere with results of some HbA1c estimation methods leading to false results and this may have negative clinical sequences. Materials and Methods: This study is a laboratory based cross-sectional study, carried out during the period from September 2017 - July 2018 in Khartoum State. Blood samples were collected from a total of 82 diabetic patients who attended the Modern Medical Center for regular follow up; 41 of them with poor control (high HbA1c) and 41with good control (normal HbA1c). Adult diabetic patients with conditions known to affect Hb-F level such as Haemoglobinopathy, Leukaemia and Hereditary persistence of fetal haemoglobin were excluded from the study. Data was collected by a structured questionnaire and analyzed by a statistical package for social sciences (SPSS), version 21. This study was approved by the scientific research committee, Faculty of medical laboratory sciences, Al Neelain University and verbal consent was obtained from each participant. HbA1c was measured by enzymatic method and Hb-F level was measured by capillary electrophoresis.
FLT3 - Internal Tandem Duplication and C-kit D816V Mutations in Sudanese Patients with Acute Myeloid Leukaemia
(جامعة النيلين - كلية الدراسات العليا, 2017-01-16) Nada Daoud Elzubeir; Leena Babiker Mirghani; Elshazali Widaa Ali
Background : There are considerable data showing that AML, like other human cancers, is the consequence of more than one mutation. ﬁns-like tyrosine kinase 3 (FLT3) and KJT genes belong to the family of tyrosine kinase class HI receptors that induce signals for cell proliferation. Mutations of these genes; however, result in autonomously leukemic cell proliferation and an unfavorable prognosis. Objectives: To determine the frequency of C-kit D8l6V and FLT3 ITD mutations among Sudanese patients with AML. Material and method: This study included 44 newly diagnosed AML Sudanese patients. Two and half milliliter (ml) of venous blood were collected from each patient in EDTA container for hematological and molecular analysis. Genomic DNA was extracted by DNA Salting out protocol . All samples were analyzed for FLT3-ITD mutation on chromosome 13, exon 11 using conventional PCR and C-kit mutation at codon 816 of exon 17 using allele speciﬁc PCR. Data was analyzed by statistical package for social sciences (SPSS), version 20. Result: A total of 44 Sudanese patients diagnosed with AML were enrolled in this study .l9(43.2%) were males and 25 (56.8%) were females ; their age ranged 2-92 years (mean 39.53). Blast percent was ranged 23-90% (mean: 54.1%) and total WBCs count ranged 2100- 28000/L (mean: 1631). The results showed that, while FLT3-ITD mutation was totally absent, C-kitD816V mutation was foimd in 50% of the patients. No statistically signiﬁcant difference was found in mean age of incidence (P. value = 0.974), blast percentage (P.value = 0.595), and total WBCs count (P. value = 0.123). The results showed no statistically signiﬁcant correlation between C- kitD8l6V mutation and each of gender ( P.value = 0.761) and subclass 0fAML (P. value = 0.818). Conclusion: C-kit D8l6V mutation was found in half the Sudanese patients with AML while FLT3 —ITD mutation was totally absent. C- kit mutation has no effect on age of incidence, blast percentage, and total WBCS count, and has no correlation with gender and subclass of AML.
Molecular and Hematological Characterizations of Acquired Alpha Thalassemia among Sudanese Leukemic Patients
(Alneelain University, 2014) Leena Babiker Mirghani
Abstract Background Acquired alpha thalassemia (hemoglobin H (I-lbH) disease) is a rare complication of neoplastic chronic myeloid disorders especially myelodysplastic syndrome and other types of leukemia. The association between alpha thalassemia and leukemia type or prognosis is deﬁned by the presence of HbH inclusion which indicated by down regulation of alpha globin gene expression. Objective This study aimed to determine the frequency of the acquired alpha thalassemia (HbH disease) among Sudanese leukemic patients and studying the hematological and molecular characteristics features of this disorder. Materials and methods This descriptive study include 135 Sudanese leukemic patients having a different type of leukemias, male and female with various age groups was enrolled in different hematological and molecular tests. One blood sample was collected in (EDTA) anticoagulated container from each patient in order to perfonn a complete blood count, HbH detection and for molecular analysis. The CBC was perfonned by automated (Sysmex 21), and leishman stained blood ﬁlms was examined to conﬁnn the type of associated anaemia. Blood ﬁlms also prepared with BCB stain were used to detect HbH containing cells. RNA was extracted from the samples with positive HbH, converted to cDNA and analyzed for alpha beta globin ratio using Real Time RT-PCR. Results: A total of 129 samples from Sudanese leukemic patients enrolled in this study for quantitation of alpha\beta ratio, HbH preparation and CBC. The frequency of the anemic patients among the population was 81.4 %, the NN anemia represents (58.9%) of the total anemic patients, the MH and MN showed 39.5%, 1.6% respectively. The frequency of HbH inclusions among the study population was 27. % positive sample, the positive HbH preparation among each type of leukemia showed a higher frequency in AML (40.6%) followed by (35.0%) in CML while showed lower results in lymphoid l