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|Title:||The Effect of Transfer Factor and BCG as Immunoprotective in Mice infected with Mycobacterium tuberculosis|
|Authors:||Jamal Baid Salim Mohammed|
|Abstract:||ABSTRACT Background: Tuberculosis, caused by Mycobacterium tuberculosis was one of the most serious diseases in Sudan and elsewhere. The disease was quite prevalent in Eastern Sudan. Objective The aim of this experimental study is to determine the protective efficacy of transfer factor (TF) as immunoprotective for mice in comparison to BCG. Materials and Methods To evaluate the immunoprotection of experimental mice by several parameters including; survival of challenged mice, mortality rate and delayed type hypersensitivity A total number of 102 mice were used. Experiment(1) n=10 for each group A and B for the susceptibility and resistance of the strains of mice by immunization of mice with BCG for 21 days and testing by tuberculin skin test (TST). experiment(2) preparation of T.F. n = 10. Experiment(3) contains three groups of mice of 10 mice n = 10 each . The first group(A) were immunized with BCG (0.2ml) first dose (I.P) for 21 days. The second group (B) were immunized with BCG (I.P) as first dose for 15 days and boosting dose (second dose) for another 15 days. The third group(C) were not immunized with BCG. All the three groups were challenged with (0.5ml)virulent M.TB. Experiment(4) Three groups of mice were used n= 12 for each group (36mice), the groups were T1,T2 andT3 .The mice of group T1and T2 were given murine transfer factor(mTF) which prepared from the spleen of infected mice and 24 hours later all the mice of groups T1and T3 were challenged with virulent M. tuberculosis H37Rv strain (American Type Culture Collection, ATCC 35718)M.TB.107(CFU).Group T2 was not challenged with MTB . Experiment(5) n=6 for the study of humoral response by immunization of mice with immune serum and challenged with M. tuberculosis H37Rv strain. Results: After three weeks of observations the mice of experiment(1) were tested for tuberculin skin test and the result were positive. Following the results of experiment(2), survival mice in group (A) were 50%, group (B) 70% and group(C) 0%.The mortality rates for (A) 50%, (B) 30% and (C) 100%.Experiment(3) results of Effectiveness determination of murine transfer factor (mTF) of the experiment as the rate of challenged survival mice wereT1(83.3%), T2(100%) and T3 was (0 %) and mortality rates wereT1(16.7%), T2 (0 %)and T3(100%). Tuberculin skin test reaction for the BCG immunized mice were positive, hence the mice strain of BALB/c were susceptible and therefore T.F can be prepared, while the strain of Swiss white mice were resistant for BCG. The immunopotency and protective efficacy of BCG first dose and boosting dose were (50%) and (70%) respectively while effectiveness determination of TF as protective efficacy was (83.3%). Humoral immunity response against M.TB .showed negative reaction hence mortality rate was 100%. Conclusions: Transfer factor (TF) was shown to be capable of transferring antigen-specific cell-mediated immunity (CMI) to T lymphocytes and therefore TF can successfully be used as treatment for M.TB. and specially for MDR-TB and supplementary for conventional chemotherapy, in which CMI plays a relevant role in protection and control of the disease, such as intracellular bacterial diseases (tuberculosis, leprosy) and parasite infections (leishmaniasis, toxoplasmosis).The incidence of TB is high inspite of primary vaccination in neonatal period and therefore requires consideration for repeated immunization of BCG. Research concerning TF are going on to discover secrets of TF in protection from diseases, increasing and transferring of immunity in patients.|
|Appears in Collections:||PHD theses : Medical Laboratory Science|
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