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Title: Calculation of argyrophilic nuclear organizer region (Ag-NOR) in Sudanese Patients diagnosed with Prostatic adenocarcinoma
Authors: Mohamed Eltayeb Mohamed Abdulbagi
Keywords: argyrophilic nuclear organizer region
keratocystic odontogenic tumor
Prostatic adenocarcinoma
Issue Date: 2019
Publisher: Al-Neelain University
Abstract: In Sudan; the incidence of prostate cancer is high and it is the most common cancer in men. Differentiating between benign and malignant lesions could be difficult especially in early stages. The difficulties could increase when result is needed in a short time and concrete diagnosis and grading is tricky. Ag-NOR is one of the stains that test the NOR regions in the chromosomes which indicates the degree of DNA expression that can differentiate cancer cells from normal cells. In this study we used Ag-NOR stain in order to test nuclear activity in normal cell compared to benign and to sort out the differences with tumor grade. Materials and Methods: This is a retrospective case control study that includes about 100 cases. Half were diagnosed with prostate adenocarcinoma and the rest are begin prostatic hyperplasia used as controls group. Details of the patients were recorded which included their age, residence and grade of cancer. The samples were then cut into sections using light microscope, each of 5micron thickness. One section was subjected to Hematoxylin and Eosin (H and E), and the second was stained with silver nitrate stain for the detection of Ag-NOR stains. Degree of differentiation was estimated and correlated with the Gleason score and the outcome of the staining. Results: The mean age of the case group was 69 years and for the control group was 61. The majority of the participants in both groups were from Khartoum state. Most of the adeoncarcinoma were grade 3. In terms of Argyrophilic Nucleolar Organiser Region (Ag- NOR) count per nucleus, the number of spot/dots in benign lesions was less than the count observed in malignant lesions per nucleus. Furthermore, the number of dots increases with the advanced grade of tumor. Discussion: The mean age case group is almost similar to the common known age of men diagnose with prostate cancer. Khartoum province is where the main cancer treatment centers are located that is why patients transfer to Khartoum for medical care. The advanced stage at diagnosis is not accommodate with prostate cancer but is possible especially with the lack of diagnostic centers affect early diagnosis. Ag-NOR count per nucleolus is higher in tumor cells than in cancer cell shows the expressive biological process that differentiate cancer cell from benign.
Appears in Collections:Masters theses : Medical Laboratory Science

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