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|Title:||Callus Induction and Antimicrobial Activities of Callus and Intact Plants Extracts of Datura slramonium and Nigella sativa ‘ BY|
|Authors:||Futooh Zaydan Abd-Al- Rahman Mahmood|
|Keywords:||Callus Induction and Antimicrobial Activities|
|Abstract:||Abstract In this study, extracts of two well known medicinal plants in Sudan, namely Datura stramonium (Solanaceae) and Nigella sativa (Ranunculaceae), and their induced callus were investigated for their antimicrobial activities against four bacteria (Bacillus subtilus , Staphylococcus aureus , Escherichia coli and Pseudomonas aemginosa) and two fungi (Candida albicans and Aspergillus niger) by using agar diffusion method. In order to induce callus, leaf explants from D. stramonium and hypocotyls and cotyledons explants from N. sativa were cultured in MS media supplemented with different types and different concentrations of growth regulators. The explants of D. stramonium initiated callus very rapidly after three weeks when grown on MS medium supplemented with 2.0 mg/l 2,4-D, while the other concentrations showed less rate of callus formation. MS medium supplemented with combination of (0.05) 2,4-D + (0.025) kinetin showed faster rate of callus initiation when compared with medium with (0.05) 2,4-D alone. Hypocotyls and cotyledons explants of N. sativa showed a rapid rate of initiation of callus aﬂer two weeks when grown on MS media supplemented with NAA at 1.0 mg/l and 5.0 mg/l of NAA respectively, while a slow rate of induction of callus observed when the hypocotyls grown on MS media supplemented with 5 .0 mg/l 2,4-D and 0.5 mg/1 2,4-D when the explants were cotyledons. The NAA in this study was found to be the suitable hormone regulator for N sativa for both types of explants used.|
|Description:||A thesis Submitted For Fulﬁllment of the Degree of M.Sc. in Biology (Plant tissue culture)|
|Appears in Collections:||Masters theses : Science|
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